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•  Breeding Journal, Species: Paracanthurus hepatus

  Change Page: < 12345678 > | Showing page 5 of 8, messages 81 to 100 of 141 - powered by ASPPlayground.NET Forum Trial Version Reports tied to this Journal Spawn: http://www.mbisite.org/SReview.aspx?ID=293 Hatch: http://www.mbisite.org/HReview.aspx?ID=198 Author Message Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re: Breeding Journal, Species: [Paracanthurus hepatus] Wednesday, April 4, 2012 1:34 PM (permalink) Good to see that they are still active. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #81 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Wednesday, April 4, 2012 7:57 PM (permalink) The eggs were hatched today. Prolarvae are doing their thing.   Im not 100% sure but this batch seem easier to spot in the water, it may be a change in this batch or that I am just getting better at spotting them.  But it was quite easy to count 15 of them in a 300ml container without a magnifying glass. Approx 40 hours post spawn   Approx 50 hours post spawn <message edited by CaptCrash on Thursday, April 5, 2012 7:12 AM>   #82 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Friday, April 6, 2012 10:41 PM (permalink) About 75 hours post spawn   #83 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, April 7, 2012 12:01 AM (permalink) Nice pic! I wish I'd had that one to use in the presentation I did last night. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #84 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, April 7, 2012 7:10 AM (permalink) Yes. Very nice! They look quite good. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #85 frannyboy Total Posts : 28 Reward points : 66 Joined: 2/23/2012 Location: Liverpool, Lancashire, GB Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Sunday, May 13, 2012 1:52 PM (permalink) Any more news?   #86 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Monday, May 14, 2012 3:31 AM (permalink) No new news of late. Im still working on culturing food items for them, so I have skipped a few weeks of spawns.   Im hoping to have some progress to report in a couple of weeks, once I have been able to source some ciliates that a local university is culturing.  At this stage it will be at least two weeks before this attempt.   #87 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re: Breeding Journal, Species: [Paracanthurus hepatus] Monday, May 14, 2012 8:01 AM (permalink) Good luck! It's easy to get distracted while learning to culture food items (I'm several years into it myself). --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #88 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Monday, May 14, 2012 8:18 AM (permalink) Originally Posted by Umm_fish? I'm several years into it myself No don't say that, its going to be simple I tell you (crosses all fingers and toes)   A friend has loaned me a microscope, so I took the opportunity tonight to play with it a bit and stitch some photos together of a few eggs. They are about 2 hours post spawn       #89 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 19, 2012 6:21 AM (permalink) With me playing with the eggs pictured above, I played with them under the microscope for too long, they all failed to develop.   So I waited for the next batch. This time my collection resulted in an estimated 500 - 600 eggs in my 5L water sample.   So this will give me an opportunity to rerun a few of my previous tests and see if I get any further with larger containers or smaller containers, different food choices.   The following videos are via the loaned microscope, 10 minutes after spawn   A question I have is why would some of the eggs have a split/line through the oil droplet and others dont.  This was taken between 10 and 15 minutes from spawn, do I would not have thought that it would be about cell division as yet.   These eggs were collected from my 5L sample by pipette and placed onto the microscope slide in a single drop of water from the pipette. <message edited by CaptCrash on Saturday, May 19, 2012 11:41 AM>   #90 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 7:28 PM (permalink) double post <message edited by CaptCrash on Saturday, May 26, 2012 9:28 PM>   #91 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 7:28 PM (permalink) With the last few clutches, I have been able to see a severe change in prolarvae development depending on temp of hatch water (and therefor time in the egg)   Typically, ambient temp has been fine for hatching the eggs, however this was through summer here in Perth.  As the ambient temp has fallen, the success rate of the eggs has been much lower and deformities have occurred in the hatched prolarvae.   By adding a heater at 27c, the development has returned to previously experienced time frames.   eg. This video is at about 28 hours post spawn.     #92 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 9:16 PM (permalink) That is too cool! http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #93 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 9:35 PM (permalink) Originally Posted by aomont Originally Posted by CaptCrash I have the link to http://www.springerlink.com/content/l118n34n1m04477j/ I am being a bit cheap and I have not purchased it as yet. All you need is a PM with your e-mail. It works for you too Luis. Just ask and it will show up Can you read the email address? I have: a02111u@cc.miyazaki-u.ac.jp, but the first 2 digits are fuzzy to me.   #94 aomont Total Posts : 774 Reward points : 1003 Joined: 2/12/2011 Location: Rio de Janeiro, RJ, BR Status: offline MBI: 5 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 10:36 PM (permalink) I don't know if I understood, Kathy. I just told them to pass me their e-mail because I have the paper and could send it to them. Do you want it too ? <message edited by aomont on Sunday, May 27, 2012 12:39 AM> Anderson.   #95 aomont Total Posts : 774 Reward points : 1003 Joined: 2/12/2011 Location: Rio de Janeiro, RJ, BR Status: offline MBI: 5 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Saturday, May 26, 2012 10:36 PM (permalink) Double post <message edited by aomont on Sunday, May 27, 2012 12:41 AM> Anderson.   #96 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Friday, June 1, 2012 12:00 PM (permalink) Latest batch of pro-larvae With higher temps, the problem of deformed pro-larvae seems to have disappeared but this has had a detrimental affect on the eggs hatch rate, with a major increase in the number of cilliates feasting on eggs.  I dont know if the cilliates are causing the egg failures or are just taking advantage of them.       #97 fla2341 Total Posts : 4 Reward points : 86 Joined: 5/22/2012 Location: debary, FL, US Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Sunday, June 3, 2012 11:23 PM (permalink) CaptCrash I know it's not the same species but I can attribute at least 3 things which effect the eggs development/not developing in my Flame Angels: 1. PH - I dose Kalk and found that if some of the kalk was acidently dumped into the tank 2-3 hrs prior to spawn I got eggs which only developed so far or malformed pro larvae. I figured that out a couple days ago when someone "helped me" and put about 1/2 gallon of kalk water into the main tank, "cause the sump looked low", then set up a drip - in the main tank and not the sump.   The pH must have risen, then was kept higher then normal at the surface because the kalk wasn't able to mix in the sump 1st and my surface agitating pumps turn off at 8pm.  2. Temp - I keep the breeding pair at 79-82 degrees F.  The eggs at 80-82 degrees F.  Any colder then 80 degrees and the eggs take much longer to develop.  Colder prolonged temps of 75 or less and nothing happens - no development.  Prolonged temps hotter then 84 degrees F and they develop to prolarvae but 90-100% die shortly after. 3. Unfertilized eggs which still float for 15+ hrs.  I had a couple spawns where the male missed? and only a few eggs ended up fertilized.  This happens when the pair are disturbed around the spawning time.  The fertilized eggs developed but these eggs did not.  They remained clear & floating until I removed them. All other spawns are 90%+ fertile.   I've also experienced delayed development in some eggs- as much as 4-5 hours delay - while the other eggs in the same spawn developed as previously, some eggs (only a handfull) had a delayed development and hatching.   Cilliates: I think this is opportunistic feeding on non vital eggs.   #98 CaptCrash Total Posts : 660 Reward points : 1296 Joined: 10/23/2011 Location: Perth, WA, AU Status: offline MBI: 2050 Pnts MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: [Paracanthurus hepatus] Monday, June 4, 2012 1:19 AM (permalink) Originally Posted by fla2341 I know it's not the same species but I can attribute at least 3 things which effect the eggs development/not developing in my Flame Angels:  1. PH - I dose Kalk and found that if some of the kalk was acidently dumped into the tank 2-3 hrs prior to spawn I got eggs which only developed so far or malformed pro larvae.   You may be onto something there, my PH was low at the time of the two batches that were deformed.  Originally Posted by fla2341   2. Temp - I keep the breeding pair at 79-82 degrees F.  The eggs at 80-82 degrees F.  Any colder then 80 degrees and the eggs take much longer to develop.  Colder prolonged temps of 75 or less and nothing happens - no development.  Prolonged temps hotter then 84 degrees F and they develop to prolarvae but 90-100% die shortly after.   When I added the heaters I set them at 27c/80f, the same batch also had a container with non-heated water that was at room temp, 24-25c. None of the eggs in the unheated container hatched. Some of the eggs in the heated container hatched (40-50% with a mix of eggs from both females) The majority of the eggs in a heated and lit 12 hours per day container hatched (40-50% with a mix of eggs from both females)   I think the combination of the PH being low, temp and lighting have all played a part in my latest batches failing. Also when the prolarvae are hatching, the oil globule appears smaller than some of my earlier batches.   Originally Posted by fla2341   3. Unfertilized eggs which still float for 15+ hrs.  I had a couple spawns where the male missed? and only a few eggs ended up fertilized.  This happens when the pair are disturbed around the spawning time.  The fertilized eggs developed but these eggs did not.  They remained clear & floating until I removed them. All other spawns are 90%+ fertile.   Up until the last month or six weeks, I get really good hatch rates from my larger female (also larger eggs, about 0.5mm diameter) My smaller female, I have been getting on and off fertilisation (50% or so) (smaller eggs 0.3mm diameter)   With the tangs, the fertilised eggs continue to float, either at the water surface or just below (less than an inch).  Unfertilised eggs tend to sink quickly and/or turn opaque with Cilliates.  The white/opaque is the cilliates in massive numbers, the egg remains clear, until it goes bag/rots and then changes to a brown/red colour. I now try to remove any coloured eggs after about 12-24 hours.  Originally Posted by fla2341   I've also experienced delayed development in some eggs- as much as 4-5 hours delay - while the other eggs in the same spawn developed as previously, some eggs (only a handfull) had a delayed development and hatching.   I see some variability, but I haven't actually sorted or anything like that, other than if they go bad.   Originally Posted by fla2341   Cilliates: I think this is opportunistic feeding on non vital eggs. Yes, this is defiantly the case, they are present on the developing eggs eg http://www.mbisite.org/Fo...1854&mpage=1#71884 and in various forms, but dont seem to damage the eggs or be present in anywhere near the same numbers. For non developing eggs, its a different story they swarm.   Thanks for all of the information, I think its really good to know.  Even though some of the things are quite different, some are very similar <message edited by CaptCrash on Monday, June 4, 2012 8:03 AM>   #99 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re: Breeding Journal, Species: [Paracanthurus hepatus] Monday, June 4, 2012 5:50 AM (permalink) Originally Posted by aomont I don't know if I understood, Kathy. I just told them to pass me their e-mail because I have the paper and could send it to them. Do you want it too ? Thanks Aomont! Yes, I would like the paper.   kathysclowns@sbcglobal.net   #100 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 12345678 > | Showing page 5 of 8, messages 81 to 100 of 141 - powered by ASPPlayground.NET Forum Trial Version

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