Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis
Species description: Reef Culture -
http://www.reefculture.com.au/store/catalog/rotifer Culture source (link if possible
): If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 11/16/2011 (16 November 2011)
Continuation Date: ongoing, report for 90 days is 2/14/12
Culturing Vessel Details Salinity: 1.020
Temperature: not measured
pH: not measured
Vessel description: 20L white Salt Bucket with approx 15L of water/rotifers and Reed Rotifer Diet.
Lighting description: None. Lid is on/ajar
Lighting cycle: None
Aeration description: Solid airline bubbling at a fast rate, too fast to count
Methodologies Split methodology: Once the density of Rotifers peaks (or two weeks), the culture is moved to a new set of buckets. Or a new single bucket and 50% are frozen depending on demand.
Both buckets are filled to 15L mark with new aged artificial salt water and provided with Rotifer Diet to colour water deep green. The Rotifers from the old bucket are filtered through a series of screens and the contents remaining on the 53um screen are back washed into the two new buckets.
Culture medium description: Rotifers are feed with Reeds Rotifer Diet at a level where by the bottom of the container cannot be seen.
This is in the order of 100ml - 200ml of diluted Rotifer Diet per 2 days. One part rotifer diet is diluted with 10 parts aged artificial salt water.
Cell count: Not measured
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: 2 x 500ml initial packages of rotifers were used to start a culture (1 leaking, giving 2 x 300ml starters).
as of 4th December 2011, 3 x 15L cultures are in heavy density and 2 x 15L cultures are light density (new start)
Weekly the "muck" on the bottom is siphoned out.
Daily remove 1.5L per day of liquid and replace with new aged artificial salt water. Rotifers are used to feed to fry or are frozen depending on demand.
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<message edited by CaptCrash on Monday, February 20, 2012 8:58 PM>