Culture Journal, Species: Isochrysis galbana

General
Species:  Isochrysis galbana
Species description:  Brown algae
Culture source (link if possible):  http://florida-aqua-farms...;product=LIVE_CULTURES
If algae, CCMP # (Optional):  CCMP 462, 1323, 1611
http://ccmp.bigelow.edu/
Culture Establishment Date:  12/12/2011
Continuation Date: 

Culturing Vessel Details
Salinity:  1.019
Temperature:    80F
pH:  8.0

Vessel description:  right now, the dish it came in, moving to a 2 qt glass jar.
Lighting description:  PC lighting. 72" fixture four bulb
Lighting cycle:  on 10:00am off 10:00pm
Aeration description: none yet, slow rolling bubble when in a bottle

Methodologies
Split methodology: none yet

Culture medium description:  1ml f/2 daily 
 
Cell count:  unknown
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes:  going to put this in a cooler spot. Trying to keep around 70F after it's established.



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If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.
 
 
 

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

Good luck with this.  I've never heard of a FAF disk working for Iso, but if you have success, send some down my way.  I could also use some O marina.  Mine has turned to ciliates, as has one of my A panamensis.  I have another, however...
 
I think the problem may stem from Iso being a dinoflagellate, and it can't move on a disk.

I'm definitely coming up this Sunday. I'll call you and meet up before I go do my shopping.

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

still just a light brown. They said it can take up to a week to start really growing.
 

 

 

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

That looks pretty good.
 
I've restarted mine again from the dying culture, after I bought fresh fertilizer from FAF, and I 'm very pleased.  I still could use some O. marina, and some M. salina if you can spare it.

bringing it tomorrow. You're my first stop. I'll know a time frame later and let you know. I just hope there's enough to restart for you.

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

Have you gotten the culture established?

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

This is as far as it's gotten. It's still got a brown tint to it, but it's not to dark. I might just get some live for another culture and keep trying to get this one going.

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

Any updates?

How about now? any updates?