Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis Species description: L or S strain of marine rotifer Culture source (link if possible
): TCMAS club member screen name patent
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 12/9/2011 restarted 12/31/11: restarted again on 1/17/13
Continuation Date: ~ 3/9/2012 (new continuation date: ~ 4/17/13)
Culturing Vessel Details Salinity: ~1.020
Temperature: ambient room temp (65 - 72 degrees F)
pH: not observed ~ 7.8 - 8.2
Vessel description: black polyethylene 5 gallon bucket
Lighting description: ambient from south window about 10 - 15 feet away
Lighting cycle: ambient
Aeration description: ridget air tube fastened to a piece of live rock at a rate of 2-3 bubbles/sec Methodologies Split methodology: syphon off 1/4 of bucket volume through a 53 um sieve and return water to bucket.
Culture medium description: salt water at approx 1.020 with RG+ supplimented with live green nanochloropsis enough to make it so that the bottom of the bucket cannot be seen.
Cell count: unknown
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by reeflover on Saturday, March 16, 2013 1:22 AM>