Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Nannochloripsis
Species description: Micro Algae Culture source (link if possible
): http://florida-aqua-farms.com If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: November 2, 2011
Continuation Date: 4/12/2012
Culturing Vessel Details Salinity: 1.020
Temperature: Ambient ~72
pH: 8.0
Vessel description: 7 2 liter bottles.
Lighting description: 2 fluorescent 4' tubes mounted horizontally
Lighting cycle: 16 hours on, 8 off.
Aeration description: Firm bubbling with stiff tube. Not terribly fast, but not a trickle.
Methodologies Split methodology: When its really, really green (While I do track how old a culture is, I usually just take the darkest culture that day), I take 1/3 and pour into Rots or 1/3 into rots and 1/3 into greenwater in evening. If still have 2/3 left, pour other 1/3 into rotifers in morning, refill with culture medium, and replace. Periodically change out 2 liter bottles.
Culture medium description: Saltwater with IO salt at 1.020 salinity, add 2 ml of Florida Aqua Farm micro algae grow fertilizer (liquid) to one gallon prep culture.
Cell count: unknown. Do measure with FAF secchi disk, measures to 61.4 million/ml, but no way to calibrate device to be sure its accurate.
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by patent on Sunday, May 20, 2012 7:58 PM>