Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis
Species description: L strain Rotifers
Culture source (link if possible
): from a friend - unsure where they got it from
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 17.12.11
Continuation Date: 17.03.12
Culturing Vessel Details Salinity: 1.020
Temperature: in the kitchen window sill, so room temperature possible fluctuates
pH: not tested
Vessel description: 5 ltr glass demijohn
Lighting description: natural day light, lights switched on as kitchen get used
Lighting cycle: natural
Aeration description: air pump, fast bubble rate
Methodologies Split methodology: dont really split unless im increasing the number of containers, so far have done 90% havests and built back up from there, and syphon a few inches from the bottom every other day topping up with fresh water. feeding twice daily with reef phyto concentrated roti rood, and once weekly a few mls of culture conditioner is added to each demijohn
Culture medium description: 3 x 5 ltr demijohn with salt water at 1.020sg containing rotifer cultures
Cell count: not known
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: first attempt at any culturing anything, hopefully will lead to being able to set up a small breeding setup
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by matt1001 on Monday, November 12, 2012 7:10 PM>