Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Isochrysis galbana
Species description: Inoculation of AlgaGen's PhycoPure copepod blend, containing Isochrysis galbana and
Chaetoceros diatom
Culture source (link if possible
): http://www.algagen.com/prod/?p=301 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 12/5/2011
Continuation Date: Culturing Vessel Details Salinity: 1.020
Temperature: Ambient, about 75 degrees F
pH: unmeasured
Vessel description: Florida Aqua Farms culture bags: 7.5 L square bottom bag, 6 inch x 4 inch x 22 inch. Each bag holds about 3 liters in my phytoplankton culture station.
Lighting description: Two 23 watt Soft White CFLs for two bags
Lighting cycle: 16 hours on
Aeration description: A good "boiling" aeration (no stone)
Methodologies Split methodology: Once a week, if the culture is dark brown, I take one bag and half goes into the refrigerator for feeding parvocalanus and rotifers. I fill the bag back up with sterilized artificial seawater.
Culture medium description: I use "f strength" dose of MICRO ALGAE GROW from Florida Aqua Farms. I sterilize the artificial seawater with bleach and then dechlorinator.
Cell count: I can't accurately measure it. It's pretty dark golden-brown.
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Although I started with the Iso and the diatom
Chaetoceros in my culture, I have not found any sign of the diatom under microscopic examination of the culture.
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