Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis
Species description: Green motile alga (I think?)
Culture source (link if possible
): fellow hobbyist gave me about a 1 1/2 cup starter
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: Mar 18, 2012
Continuation Date: Jun 18, 2012
Culturing Vessel Details Salinity: 1.020
Temperature: Ambient temperature is 78-80F in the culture cabinet.
pH: Not measured
Vessel description: Drinking glasses for now. Will buy some 2L soda bottles (I don't normally drink it).
Lighting description: 2x 13 watt T5 fluorescent 3100K
Lighting cycle: 18 hours on, 6 hours off
Aeration description: Almost violent. Will make an inline air filter asap.
Methodologies Split methodology: I'm told to split it every 10 days.
Culture medium description: Waiting for Guillard's f/2 to arrive from FAF. Currently using 2 liters RODI saltwater, 5 mL Miracle Grow liquid, 1 mL Kent Essential Elements.
Cell count: Reference links: Additional Information Notes: This is my very first try culturing phytoplankton, so PLEASE make suggestions if I'm not doing something right!
<message edited by EasterEggs on Tuesday, July 10, 2012 5:28 PM>