Culture Journal, Species: Tetraselmis (FAF) (Failed)

General
Species:  Tetraselmis
Species description:  green, motile algae
Culture source (link if possible):  Florida Aqua Farms
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  03/23/2012
Continuation Date:  On going (hopefully)

Culturing Vessel Details
Salinity:  1.015
Temperature:  Not measured  (Fans turn on at 20c to cool algae cupboard)
pH:  Not measured
Water Details: Artificial Salt with RO/DI water.

Vessel description:  2000ml Conical Flask
Lighting description:  CF light
Lighting cycle:  16 hours per day
Aeration description: Heavy Aeration, adjusted to rotate culture approx 1 per second.

Methodologies
Split methodology:  Cultures will be split, 500ml to one or two new flasks, remainder to fridge to feed as required. Split will occur depending on dark green colour/density of culture.

Culture medium description: 
RO/DI water with Artificial Salt to 1.015.  Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.8ml / 2000ml salt water.

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
03/23/2012 two separate FAF disks were initiated to provide two algae sources.
03/30/2012 two separate flasks were combined, assumption was that they were not going to be viable

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Both disks look to have failed.
All that I have is some brown sediment in the bottom of the container.
Im letting it go, to see if something comes of it.
My guess is that the disks were too old, I have had them for a few months now.

I have left this going and it may prove to be a good choice.  The water is looking better today.
Both cultures were added to a single flask and it was topped up to 2000ml with new culture water (300ml).

Seems to be continuing to get better.
 
See back left flask

 
The only thing weird about this at the moment, is there is quite a bit of filament algae growing in this flask.
The front three flasks are also Tetraselmis but from a different source (CSIRO).
The FAF Tet, was initiated over a week before the front ones and is only really now just starting to look viable (its actually a bit darker in person).

This culture failed and was disposed of on 04/11/2012
 
I expect that if I had run it through a sieve and cleaned the culture that it may have been recoverable, but as I now had another source, I didn't bother.