Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis chuii
Species description: Single cell with 4 equal flagella
Culture source (link if possible
): CSIRO Australian National Algae Culture Collection
http://www.marine.csiro.a...recid=34955&-find= If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 03/27/2012
Continuation Date: 06/25/2012
Culturing Vessel Details Salinity: 1.015
Temperature: Not measured (Fans turn on at 20c to cool algae cupboard)
pH: Not measured
Water Details: Artificial Salt with RO/DI water.
Vessel description: 2000ml Erlenmeyer Flask/Conical Flask
Lighting description: CF Light, 3 x double bulb, 37W fixtures. 4 x 5000k bulbs installed (2 spares)
Lighting cycle: 16 hours per day, 7am to 11pm
Aeration description: Heavy Aeration, adjusted to rotate culture approx 1 per second.
Methodologies Split methodology: Cultures are slit as follows, 500ml to two or three new flasks or soda bottles, remainder to fridge to feed as required. Split will occur depending on dark green colour/density of culture.
Culture medium description: RO/DI water with Artificial Salt to 1.015. Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water.
Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch. Batch is allowed to cool to room temperature before use.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: 03/21/2012 125ml of sterile source algae was ordered from CSIRO (order was for 250ml and was shared with another person).
03/27/2012 Four separate cultures established with 4 x 65ml starter cultures
04/01/2012 2 x 400ml cultures and 1 x 125ml culture provided to another person.
04/15/2012 split 2
04/27/2012 split 3
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by CaptCrash on Monday, June 25, 2012 4:44 AM>