Culture Journal, Species: Nannochloris (FAF)

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CaptCrash
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Culture Journal, Species: Nannochloris (FAF) - Thursday, March 22, 2012 11:19 AM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Nannochloris
Species description:  Single, round cell
Culture source (link if possible):  Florida Aqua Farms http://florida-aqua-farms...;product=LIVE_CULTURES
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  03/23/2012
Continuation Date:  06/22/2012

Culturing Vessel Details
Salinity:  1.015
Temperature:  Not measured  (Fans turn on at 20c to cool algae cupboard)
pH:  Not measured
Water Details: Artificial Salt with RO/DI water.

Vessel description:  2000ml Erlenmeyer Flask/Conical Flask
Lighting description:  CF Light, 3 x double bulb, 37W fixtures.  4 x 5000k bulbs installed (2 spares)
Lighting cycle:  16 hours per day, 7am to 11pm
Aeration description: Heavy Aeration, adjusted to rotate culture approx 1 per second.

Methodologies
Split methodology:  Cultures are split as follows, 500ml to two or three new flasks or soda bottles, remainder to fridge to feed as required. Split will occur depending on dark green colour/density of culture.

Culture medium description: 
RO/DI water with Artificial Salt to 1.015.  Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water.
Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch.  Batch is allowed to cool to room temperature before use.

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
03/23/2012 two separate FAF disks were initiated to provide two algae sources.
03/30/2012 two separate flasks were combined, assumption was that they were not going to be viable
04/03/2012 culture looks to be recovering well and may be as yet viable.
04/15/2012 split 2
04/27/2012 split 3

You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.

<message edited by CaptCrash on Friday, June 22, 2012 3:03 AM>

CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Saturday, March 31, 2012 11:55 AM
This looks like it is going to be a fail.
 
The culture has not really established.  All that I am getting is green clumps in the bottom of the flask.
Ill let the culture continue for another week or two and see if it goes anywhere.
 
I think the problem is that my Algae disks had been sitting for a few months and were now bad.

CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Sunday, April 1, 2012 7:11 AM
Well its looking a bit more positive today.
The water is greening up, the clumps are multiplying.  Maybe its a go.
The two cultures were combined and the water topped up to 2000ml with additional media (400ml).

CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Tuesday, April 3, 2012 5:10 AM
Images of culture startup and progress
 
Initial culture disks

 
water added

 
After 24 hours under light

 
after about 5 days

 
after 11 days


CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Saturday, April 14, 2012 11:17 PM
A split of the flask was performed on 04/11/2012, taking 800ml from the conical flask and adding to a second 2000ml conical flask.  Both containers were then filled to 2L with new media.  Loss from the original 2000ml is due to evaporation and use for fry.

CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Friday, April 27, 2012 3:04 AM
I split all of my Nanno last night.  I probably left some of it a little long and some of the containers had gotten very dark and had then lightened. Others were still very dark.
In all cases (4 containers) I removed 2/3 of the algae and used it to feed rotifers/artemia, then topped up with new media, the remainder was stored in the fridge for use in the next few days.

CaptCrash
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Re:Culture Journal, Species: Nannochloris (FAF) - Friday, June 22, 2012 1:23 AM

Picture for report (looking a little light after split)
<message edited by CaptCrash on Friday, June 22, 2012 3:24 AM>