Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Nannochloris
Species description: Single, round cell
Culture source (link if possible
): Florida Aqua Farms
http://florida-aqua-farms...;product=LIVE_CULTURES If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 03/23/2012
Continuation Date: 06/22/2012
Culturing Vessel Details Salinity: 1.015
Temperature: Not measured (Fans turn on at 20c to cool algae cupboard)
pH: Not measured
Water Details: Artificial Salt with RO/DI water.
Vessel description: 2000ml Erlenmeyer Flask/Conical Flask
Lighting description: CF Light, 3 x double bulb, 37W fixtures. 4 x 5000k bulbs installed (2 spares)
Lighting cycle: 16 hours per day, 7am to 11pm
Aeration description: Heavy Aeration, adjusted to rotate culture approx 1 per second.
Methodologies Split methodology: Cultures are split as follows, 500ml to two or three new flasks or soda bottles, remainder to fridge to feed as required. Split will occur depending on dark green colour/density of culture.
Culture medium description: RO/DI water with Artificial Salt to 1.015. Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water.
Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch. Batch is allowed to cool to room temperature before use.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: 03/23/2012 two separate FAF disks were initiated to provide two algae sources.
03/30/2012 two separate flasks were combined, assumption was that they were not going to be viable
04/03/2012 culture looks to be recovering well and may be as yet viable.
04/15/2012 split 2
04/27/2012 split 3
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<message edited by CaptCrash on Friday, June 22, 2012 3:03 AM>