Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Isochrysis sp
Species description: Cells with 2 equal flagella and short haptonema
Culture source (link if possible
): CSIRO Australian National Algae Culture Collection
http://www.marine.csiro.a...recid=35112&-find= If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 03/27/2012
Continuation Date: 06/25/2012
Culturing Vessel Details Salinity: 1.015
Temperature: Not measured (Fans turn on at 20c to cool algae cupboard)
pH: Not measured
Water Details: Artificial Salt with RO/DI water.
Vessel description: 2000ml Erlenmeyer Flask/Conical Flask and plastic soda bottles
Lighting description: CF Light, 3 x double bulb, 37W fixtures. 4 x 5000k bulbs installed (2 spares)
Lighting cycle: 16 hours per day, 7am to 11pm
Aeration description: Heavy Aeration, adjusted to rotate culture approx 1 per second.
Methodologies Split methodology: Cultures are split, 500ml to two or three new soda bottles, 500ml to a new clean flask. Split depends on dark colour/density of culture.
Culture medium description: RO/DI water with Artificial Salt to 1.015. Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water.
Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch. Batch is allowed to cool to room temperature before use.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: 03/21/2012 125ml of sterile source algae was ordered from CSIRO (order was for 250ml and was shared with another person).
03/27/2012 Cultures established with 4 x 65ml starters
04/01/2012 2 x 400ml starters plus 1 x 125ml starter provided to another person.
04/15/2012 split 2
04/27/2012 split 3
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If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by CaptCrash on Monday, June 25, 2012 4:29 AM>