Culture Journal, Species: Brachionus rotundiformis

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CaptCrash
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Culture Journal, Species: Brachionus rotundiformis - Wednesday, May 30, 2012 1:04 AM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Brachionus rotundiformis
Species description:  Small round rotifers
Culture source (link if possible):  Challenger TAFE - see Additional Information/Notes
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  05/30/2012
Continuation Date:  ongoing

Culturing Vessel Details
Salinity:  1.020
Temperature:  29C  now 28c
pH:  Not measured

Vessel description:  Salt bucket, 20L with 15L of water
Lighting description: None.  Lid is on/ajar
Lighting cycle:  None
Aeration description:  Solid airline bubbling at a fast rate, too fast to count

Methodologies
Split methodology: Once the density of Rotifers peaks (or two weeks), the culture is moved to a new set of buckets.  Or a new single bucket and 50% are frozen depending on demand.
Both buckets are filled to 15L mark with new aged artificial salt water and provided with Rotifer Diet to colour water deep green. The Rotifers from the old bucket are filtered through a series of screens and the contents remaining on the 20um screen are back washed into the two new buckets.

Culture medium description: 
Rotifers are feed with Reeds Rotifer Diet at a level where by the bottom of the container cannot be seen.
This is in the order of 50ml - 100ml of diluted Rotifer Diet per 2 days.  One part rotifer diet is diluted with 10 parts aged artificial salt water.

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
1 x 500ml initial packages of rotifers were used to start a culture (split into two cultures).
Daily remove 1L per day of liquid and replace with new aged artificial salt water.  Rotifers are used to feed to fry or are frozen depending on demand.
Starter culture was sourced from Challenger TAFE (Technical Training/Aquaculute course).  It is not commercially supplied.

You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.

<message edited by CaptCrash on Thursday, September 6, 2012 9:20 AM>

CaptCrash
  • Total Posts : 649
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Re:Culture Journal, Species: Brachionus rotundiformis - Wednesday, May 30, 2012 1:22 AM
4x magnification

10x magnification

 
40x magnification

 
Im not sure if the tail missing is caused by putting this one on the microscope slide or if there is something else being the cause (different species?).  The video below shows its activity.

Others have a tail and seem to be as I would expect.
 
<message edited by CaptCrash on Wednesday, May 30, 2012 5:35 AM>

CaptCrash
  • Total Posts : 649
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  • Joined: 10/23/2011
  • Location: Perth, WA, AU
Re:Culture Journal, Species: Brachionus rotundiformis - Wednesday, August 29, 2012 10:16 AM
Images for 60 days

5G Culture bucket  with 4G of culture

 
<message edited by CaptCrash on Wednesday, August 29, 2012 12:17 PM>