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Culture Journal, Species: [Nannochloropsis Oculata]
Tuesday, June 26, 2012 1:23 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Nannochloropsis Oculata Species description: 1 - 2 µm, non motile green algae Culture source (link if possible ): Forum sale If algae, CCMP # (Optional ): CCMP525 http://ccmp.bigelow.edu/ Culture Establishment Date: 15.03.2012 Continuation Date: 26.06.2012 Culturing Vessel Details Salinity: sg1020 Temperature: 23-26 pH: 7.4-8.2 Vessel description: DIY bio-reactor 25 liters Lighting description: 2 x 18 watt florescent bulbs (6500K) Lighting cycle: 18 on 6 off Aeration description: Single air pump - experimantal yeast based CO2 suplement Methodologies Split methodology: Monthly 20 liters of harvest, 5 liters for seed. Stored in refrigirator. Culture medium description: 20 ml. Guillard f/2 or 40 ml.NPK 8-8-8 plant fertilizer. Cell count: Unknown but colour is deep green. Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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