Culture Journal, Species: Brachionus plicatilis

Author Message
mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Culture Journal, Species: Brachionus plicatilis - Tuesday, June 26, 2012 2:12 AM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Brachionus plicatilis
Species description:  L type rotifer
Culture source (link if possible):  http://www.reefphyto.co.uk/
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  22.04.2012
Continuation Date:  21.08.2012

Culturing Vessel Details
Salinity:1.017-1.020
Temperature: 26 C   
pH:  not tested

Vessel description:  3 x 15 liter glass tanks
Lighting description:  3 x 8 watt 6500K energy savers 
Lighting cycle:  14 on 10 off
Aeration description:  DIY air lifters with medium bubble count

Methodologies
Split methodology: Density check or as needed. Reset occasionally.

Culture medium description: 
500 ml. algae added daily according to population growth.       

Cell count:
10-50 per ml.         

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.

<message edited by mmoner on Tuesday, August 21, 2012 11:10 AM>

mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Re:Culture Journal, Species: Brachionus plicatilis - Tuesday, June 26, 2012 2:27 AM

 

 

 

 

 

 

 
 

mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Re:Culture Journal, Species: Brachionus plicatilis - Tuesday, June 26, 2012 2:38 AM

 
 

mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Re:Culture Journal, Species: Brachionus plicatilis - Friday, July 20, 2012 2:00 AM
Today i see little worms crawling like freshwater planaria in the cultures. Should i worry about that, any ideas?
 

<message edited by mmoner on Friday, July 20, 2012 6:02 AM>

mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Re:Culture Journal, Species: Brachionus plicatilis - Tuesday, September 25, 2012 3:54 AM
Okay culture is still going on in two seperate tanks, the tanks with worms above are cleaned and currently not active.


 

 

 

 

 

<message edited by mmoner on Tuesday, September 25, 2012 6:02 AM>

mmoner
  • Total Posts : 114
  • Reward points : 242
  • Joined: 9/8/2011
  • Location: Bursa, Bursa, TR
Re:Culture Journal, Species: Brachionus plicatilis - Friday, November 30, 2012 8:17 AM
Hi,
The culture is still going but it seems to me; it will not get more dense than that photo above. Is this the usual density you people get in your cultures? will that be enough for a single batch of larvae in 15-20 liters daily?