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Culture Journal, Species: Brachionus plicatilis
Monday, November 26, 2012 1:49 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Brachionus plicatilis Species description: L species rotifer Culture source (link if possible ): Reef Nutrition (Reed Mariculture) If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 7 Nov 2012 Continuation Date: 05 Apr 2013 Culturing Vessel Details Salinity: 1.020 S.G. Temperature: 65*F pH: 8.4 Vessel description: 2 gallon ice cream tub Lighting description: ambient from nanno culture Lighting cycle: 12 hr on 12 hr off Aeration description: rigid airline moderate Methodologies Split methodology: at this point harvesting for fry and topping up for evaporation 10 Jan 2013 culture was split by taking approx 2 liters of old culture and sieving a further 2 liters. 1 liter of fresh live nannochloropsus was then added. Culture medium description: 2-3 ml of RotigrowComplete 2x per day or 250- 500 ml of live nanno culture. Cell count: unknown Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by GinaReef on Friday, April 12, 2013 10:07 PM>
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Re:Culture Journal, Species: Brachionus plicatilis I
Monday, November 26, 2012 2:11 AM
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Some attempts at pictures of rotifers
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Re:Culture Journal, Species: Brachionus plicatilis I
Wednesday, December 5, 2012 12:05 AM
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Re:Culture Journal, Species: Brachionus plicatilis I
Tuesday, January 15, 2013 1:00 AM
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Culture continuation. Jan 10; Culture appeared to be crashing.(few fast moving rotifer observed by microscope) My phyto/phyto paste was running short. So I did a thorough split, 8 liters down to 1 liter, and added the seived rotifers from a further 2-3 liters. I added 1 liter of fresh live Nannochloropsus and 2 days later the culture appears to be recovering.(via microscope) edit: disregard date on photo. the date needs to reset on my microscope the pictures were taken Jan 12 2013.
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Re:Culture Journal, Species: Brachionus plicatilis I
Tuesday, January 15, 2013 1:10 AM
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Nice looking rotifers! The culture does appear to be doing well now.
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Re:Culture Journal, Species: Brachionus plicatilis I
Saturday, February 2, 2013 12:59 AM
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After a collapse of both cultures, probably from over population, I've finally got the cultures back to what looks like a healthy state. The cultures cleared enough to require feeding for the first time in about 2 weeks. When I needed to revive my culture I split it and sieved the waste, adding them to the remaining culture. (This seems to be my mistake.) When this tactic didn't work, I retrieved the waste that was sitting on the floor (still very green) and checked it for live rotifers. They were present in both cultures. I cleaned both vessels and floss and refilled them (no new food added) No improvement. I tested the salinity- it was very high. I diluted both cultures with about 2 liters of RO, doubling the volume. Still no improvement. Yesterday I harvested 4-6 scoops (4" sieve) out of each culture and today I could see progress. I fed each culture about 2/3 of a liter of live nanno. edit: my notes from another thread http://www.mbisite.org/Forums/tm.aspx?m=79188
<message edited by GinaReef on Saturday, February 2, 2013 1:29 AM>
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Re:Culture Journal, Species: Brachionus plicatilis I
Saturday, February 9, 2013 1:42 AM
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Culture is still alive but I am continuing to have problems getting the density of phyto/RGComplete right. I am getting too much and then not enough and the tank clears while I am out. I made the mistake of putting a heater in the tank and most of the water evaporated out before I noticed what was happening.( the house is very dry at this time of year). The salinity was incredibly high. Fortunately I have a second tank running as a back up so I have restarted the first tank.
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