Reports tied to this Journal
Author
|
Message
|
Culture Journal, Species: Tetraselmis
Saturday, December 22, 2012 3:39 PM
( permalink)
Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Tetraselmis Species description: Green alga Culture source (link if possible ): http://www.marinecolorado...ber.php?1431-AlgaeBarn , AlgaGen If algae, CCMP # (Optional ): Culture Establishment Date: Dec 9, 2012/ 25 July, 2013 Continuation Date: Culturing Vessel Details Salinity: 1.021 Temperature: Ambient temperature is around 70°F in culture room pH: Not Measured Vessel description: One quart wide-mouthed jar with a plastic lid and airline going into the culture Lighting description: 6500k leds that are about 10 cm away from the culture. I could not find the exact wattage of the leds Lighting cycle: 14 hours on, 10 hours off Aeration description: Air pump with a pace of a bubble per second Methodologies Split methodology: Every 8 days Culture medium description: FAF Micro Algae Grow of 10 drops per quart at the beginning of each culture Cell count: (if known) TBD Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Amphispur on Thursday, July 25, 2013 4:41 PM>
|
|
Re:Culture Journal, Species: Tetraselmis
Saturday, December 22, 2012 3:59 PM
( permalink)
Split out some from the source tetra, and added a bubbler. Here is it a week later:
|
|
Re:Culture Journal, Species: Tetraselmis
Saturday, December 29, 2012 2:20 PM
( permalink)
FWIW, I think that using only 3ml to start a 1L culture is too little, and runs too much risk of contamination from other sources. Frank Hoff in the "Plankton Culture Manual, Sixth Edition" says on Page 56 that, "A rule of thumb in inoculation is never to dilute more than 100-fold." He goes on to say on Page 62, in the troubleshooting section, "Too low an inoculant per volume or other factors mentioned allow bacteria to proliferate and often subdue a new culture."
|
|
Re:Culture Journal, Species: Tetraselmis
Saturday, December 29, 2012 2:23 PM
( permalink)
Thanks for the advice Jim! I will immediatly change how much the splits are. I think I will do around 1/4 of a quart.
|
|
Re:Culture Journal, Species: Tetraselmis
Sunday, December 30, 2012 1:17 PM
( permalink)
Today I am making two more batches of tetra. Currently the culture is a mixture of tetra and nano. I made two more jars for them, but I only put 8 drops of the FAF microalgae grow and I put them in the microwave for 15 minutes
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 12:16 AM
( permalink)
Hey Amphispur, Was the original culture contaminated or did that occur later?
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 9:04 AM
( permalink)
I believe that the culture started out contaminated.
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 10:20 AM
( permalink)
this is just the experience i have had but if there is NANNO in your TETRA you are going to end up with NANNO as your final result. NANNO seems to be way more aggressive than the other phytoplankton's we readily culture in our hobby. actually just had this happen to my TETRA cultures and am having to order some new ones from FAF as we speak but all this is just my exp. some of the more advanced breeders on the site might chime in and say that im completely wrong
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 7:33 PM
( permalink)
Would it be possible to refrigerate a bottle (taller the better) with the mix of algaes? Tetraselmis is motile while nannochloropsis isn't. So you may be able to take a small, dilute culture from the top of the bottle.
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 7:50 PM
( permalink)
Certainty worth a shot if anything. I've got enough of it. Now one thing I am noticing is a fine layer of green at the bottom of the jar. Is that tetra or nano?
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 10, 2013 7:53 PM
( permalink)
So I still have the original bottle, and it's been in the fridge for long enough... Lets see if I can get tetra! Here's a pic of the bottle:
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, January 17, 2013 10:28 PM
( permalink)
How's the progress? I ordered new starters, so I may be able to help you get another tetra starter.
|
|
Re:Culture Journal, Species: Tetraselmis
Friday, January 18, 2013 10:53 AM
( permalink)
Good News! After the experiment with the top of the bottle, I now have 1 culture of Tetra going! Thanks for the idea Helix
|
|
Re:Culture Journal, Species: Tetraselmis
Thursday, July 25, 2013 4:43 PM
( permalink)
Well, I never really put much into this culture after awhile, and I'm ready to start again. I got a new starter from AlgaGen: I'll be splitting this every 8 days and be somewhat treating this as my Isochrysis galbana.
|
|
|