Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Parvocalanus crassirostris
Species description: Calanoid copepod
Culture source (link if possible
): Thoughtful gift from Dr. Andrew Rhyne
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 1/8/2013
Continuation Date: 4/8/2013
Culturing Vessel Details Salinity: 1.020 - 1.026
Temperature: 72-74 F
pH: Not measured
Vessel description: Various, but mostly 2L borosilicate media jars, and 1000 mL Erlenmeyer flasks
Lighting description: Ambient fish room light.
Lighting cycle: Typically 18 hours on / 8 hours off.
Aeration description: Very light. Rigid airline set near the bottom, and about 1-2 bubbles per second.
Methodologies Split methodology: Various. See notes in Journal.
Culture medium description: New, aged artificial salt water. Fed live phytoplankton, usually a monodiet of Isochrysis, very sparingly and often. See notes in Journal.
Cell count: Varies as population of nauplii vs. adults varies over time.
Reference links: http://bit.ly/Xxlkbe http://www.sciencedirect..../pii/S0044848603001613 Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by JimWelsh on Tuesday, May 14, 2013 11:54 AM>