Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Chaetoceros muelleri (aka C. gracilis)
Species description: (Taken from Seahorse Source) This is a diatom and requires f/2 media + silica as a feed. It favors hi-light. It contains other fatty acids such as ARA and has been a standard in shrimp and bivalve farming. Also a hearty cell. Temperature range: 65º-81º F (18º-27º C). Approximate cell size: 4-10 µ wide by 4-9 µ long. Culture source (link if possible
): Seahorse Source
http://www.seahorsesource...p/detail.cgi?id=201129 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 1.24.2013
Continuation Date: 4.24.2013
Culturing Vessel Details Salinity: 1.018-1.020 SG
Temperature: Room temp, between 68 and 72 F in the winter, warmer in the summer. Cultures kept near window
pH: Not measured
Vessel description: 2L soda bottles
Lighting description: 2 bulb fluorescent shop light and natural sunlight from a southern facing window.
Lighting cycle: 6:30a to 9p
Aeration description: Rigid airline, not very vigorous
Methodologies Split methodology: Diluted starter culture 1:3 in a single 2L bottle. On day 4 it was split into two 2L bottles which were then filled with sterile, fertilized water. Cultures are split once they reach maximum darkness (gauged by eye). If a culture does not darken up as expected, half is taken and disposed of and new sterilized, fertilized media is added.
Culture medium description: 500-600uL microalgae grow per L, 6 "drops" of liquid silicate from FAF per L (
http://florida-aqua-farms...id-silicate-solution/) Cell count: Not measured
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Water is taken from broodstock tanks and sterilized with 1/2mL per L of bleach for minimum of 1hr, then neutralized with 1.5 mL per L of 1M sodium thiosulfate.
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<message edited by shannpeach on Thursday, May 30, 2013 9:01 AM>