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Culture Journal, Species: Nannochloropsis
Tuesday, March 5, 2013 6:07 PM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Nannochloropsis Species description: Culture source (link if possible ): Florida Aqua Farms (starter disk) If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 3/5/13 Continuation Date: 6/6/13 Culturing Vessel Details Salinity: 1.020 Temperature: 69-70 degrees F (ambient room temperature) pH: not measured Vessel description: 1 quart plastic Gatorade bottles, 2 liter soda bottles Lighting description: 1 x 13watt spiral CFL Lighting cycle: 16 hours on, 8 hours off Aeration description: rigid plastic tube Methodologies Split methodology: As culture matures (8-10 days at my current room temp), 1/3 of bottle used to start new culture, 1/3 used as food (presently for my coral frag tank), and then the bottle topped off with new salt water (1.020 SG). Cultures fertilized with Guillard's at standard f/2 dosing (per bottle instructions). Culture medium description: New saltwater (Instant Ocean or Reef Crystals mix) at 1.020 SG, made with tap water. No additional bleach added for sterilization. Dechlorination is done with AmQuel just prior to use, with Guillard's added at that time as well (10 drops per liter). Cell count: not measured at this time Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Lrood on Thursday, June 20, 2013 8:47 AM>
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Re:Culture Journal, Species: [Insert Species Name Here]
Tuesday, March 5, 2013 6:09 PM
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Ok, I've taken the plunge to start culturing phyto. I am hydrating my culture disk today, will place into the culture bottle tomorrow. Hopefully this time next week I have a nice green bottle to split.
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Re:Culture Journal, Species: [Insert Species Name Here]
Tuesday, March 5, 2013 7:04 PM
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Good luck, hope that you will have better success than my only partial success at starting a culture.
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Re:Culture Journal, Species: [Insert Species Name Here]
Friday, March 15, 2013 3:10 PM
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The first culture split today. The top photo was of the initial culture start-up from the FAF disk. The bottom is the new 2 liter culture. I'm going to just keep a few bottles running at present, since I'm not feeding any rotifers right now.
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Re:Culture Journal, Species: [Insert Species Name Here]
Monday, May 6, 2013 9:08 PM
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Cultures continue to grow well. I'm currently keeping 4 bottles active at a time, starting a new bottle about every 3 days. My frag tank gets the harvest at that time.
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Re:Culture Journal, Species: [Insert Species Name Here]
Monday, May 6, 2013 9:39 PM
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Nice looking cultures!
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Re:Culture Journal, Species: [Insert Species Name Here]
Thursday, June 6, 2013 9:30 AM
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Day 94 as I count them. Continuing to maintain 4 bottles, new one started approximately every 2-3 days (using about 1/3 of the oldest bottle). Using 12-15 drops f/2.
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