Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Nannochloropsis
Species description: Culture source (link if possible
): Florida Aqua Farms (starter disk)
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 3/5/13
Continuation Date: 6/6/13
Culturing Vessel Details Salinity: 1.020
Temperature: 69-70 degrees F (ambient room temperature)
pH: not measured
Vessel description: 1 quart plastic Gatorade bottles, 2 liter soda bottles
Lighting description: 1 x 13watt spiral CFL
Lighting cycle: 16 hours on, 8 hours off
Aeration description: rigid plastic tube
Methodologies Split methodology: As culture matures (8-10 days at my current room temp), 1/3 of bottle used to start new culture, 1/3 used as food (presently for my coral frag tank), and then the bottle topped off with new salt water (1.020 SG). Cultures fertilized with Guillard's at standard f/2 dosing (per bottle instructions).
Culture medium description: New saltwater (Instant Ocean or Reef Crystals mix) at 1.020 SG, made with tap water. No additional bleach added for sterilization. Dechlorination is done with AmQuel just prior to use, with Guillard's added at that time as well (10 drops per liter).
Cell count: not measured at this time
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Lrood on Thursday, June 20, 2013 8:47 AM>