Culture Journal, Species: Nannochloropsis

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Lrood
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Culture Journal, Species: Nannochloropsis - Tuesday, March 05, 2013 6:07 PM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Nannochloropsis
Species description: 
Culture source (link if possible):  Florida Aqua Farms (starter disk)
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  3/5/13
Continuation Date:  6/6/13

Culturing Vessel Details
Salinity:  1.020
Temperature:    69-70 degrees F (ambient room temperature)
pH:  not measured

Vessel description:  1 quart plastic Gatorade bottles, 2 liter soda bottles
Lighting description:  1 x 13watt spiral CFL
Lighting cycle:  16 hours on, 8 hours off
Aeration description:  rigid plastic tube

Methodologies
Split methodology: As culture matures (8-10 days at my current room temp), 1/3 of bottle used to start new culture, 1/3 used as food (presently for my coral frag tank), and then the bottle topped off with new salt water (1.020 SG).  Cultures fertilized with Guillard's at standard f/2 dosing (per bottle instructions).

Culture medium description: 
New saltwater (Instant Ocean or Reef Crystals mix) at 1.020 SG, made with tap water.  No additional bleach added for sterilization.  Dechlorination is done with AmQuel just prior to use, with Guillard's added at that time as well (10 drops per liter).  

Cell count:
 not measured at this time 

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.
<message edited by Lrood on Thursday, June 20, 2013 8:47 AM>

Lrood
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Re:Culture Journal, Species: [Insert Species Name Here] - Tuesday, March 05, 2013 6:09 PM
Ok, I've taken the plunge to start culturing phyto.  I am hydrating my culture disk today, will place into the culture bottle tomorrow.  Hopefully this time next week I have a nice green bottle to split.

Marinesciencemaniac
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Re:Culture Journal, Species: [Insert Species Name Here] - Tuesday, March 05, 2013 7:04 PM
Good luck, hope that you will have better success than my only partial success at starting a culture.

Lrood
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Re:Culture Journal, Species: [Insert Species Name Here] - Friday, March 15, 2013 3:10 PM
The first culture split today.  The top photo was of the initial culture start-up from the FAF disk.  The bottom is the new 2 liter culture.  I'm going to just keep a few bottles running at present, since I'm not feeding any rotifers right now.



Lrood
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Re:Culture Journal, Species: [Insert Species Name Here] - Monday, May 06, 2013 9:08 PM
Cultures continue to grow well. I'm currently keeping 4 bottles active at a time, starting a new bottle about every 3 days. My frag tank gets the harvest at that time.


JimWelsh
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Re:Culture Journal, Species: [Insert Species Name Here] - Monday, May 06, 2013 9:39 PM
Nice looking cultures!

Lrood
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Re:Culture Journal, Species: [Insert Species Name Here] - Thursday, June 06, 2013 9:30 AM
Day 94 as I count them. Continuing to maintain 4 bottles, new one started approximately every 2-3 days (using about 1/3 of the oldest bottle). Using 12-15 drops f/2.