Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tisbe biminiensis
Species description: Small harpacticoid copepod Culture source (link if possible
): LiveAquaria -
http://www.liveaquaria.com/product/prod_display.cfm?c=747+3137+3058&pcatid=3058&siteid=20&&topcatid=2190 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 3/4/2013
Continuation Date: Culturing Vessel Details Salinity: 1.022
Temperature: Room temp, 68-70F
pH:
Vessel description: 5 gallon inverted carboy
Lighting description: Ambient
Lighting cycle: Aeration description: Medium to Light, not enough to cause a "boil"
Methodologies Split methodology: No scheduled splits. Population reduction happens when I harvest (as needed) and when I attempt to clean detritus from the bottom of the culture. When harvesting I run the culture water though a 53um sieve. I replace this water with fresh (aged) ASW (Instant ocean).
Culture medium description: 150ml of mixed homegrown phyto (nanno and iso, 1/2 part of each) every two to three days. I also add ammonia locking additives every once in a while.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: One thing that I am trying with my tisbe culture is to use sponges (most often filter replacement sponges) to try to get culture densities higher. Since these are harpacticoid, I would assume that a 5 gallon carboy wouldn't be the best culture vessel for these. That is the problem that I hope the sponges help correct. I'm hoping that they will colonize in the sponges as well as the sides/bottom of the carboy. I also hope to use these sponges for harvesting. If colonized, I should be able to lift a sponge out, place a sieve under, and collect a good number of tisbe.
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<message edited by nrbelk on Friday, April 12, 2013 11:56 PM>