Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Nannochloropsis
Species description: green, non-motile, 4-6 microns
Culture source (link if possible
): FAF Nannochloropsis microalgae disk,
http://florida-aqua-farms.com/shop/microalgae-disks/ If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 4/15/2013
Continuation Date: 7/24/2013
Culturing Vessel Details Salinity: 1.020
Temperature: Room temperature (approximately 68-72), warming during spring/summer
pH: Not measured
Vessel description: 2L soda bottle
Lighting description: Ambient light from southern facing window, 2-bulb fluorescent strip light
Lighting cycle: Strip light is turned on from 6-9pm when days are shorter, not turned on during the summer
Aeration description: rigid airline tubing, rolling bubbles
Methodologies Split methodology: 2/3 of the bottle is taken about every week, depending on how dark the culture is. Water is replaced with sterilized broodstock tank water that has been fertilized with FAF Microalgae Grow. Then switched to using sterilized freshly mixed ASW since it is easier to prepare.
Culture medium description: 1/2mL FAF F/2 per Liter of sterlized water
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Water is sterilized using bleach and dechlorinated using sodium thiosulfate.
I actually started this culture in mid-February, but didn't get around to making the journal until now.
I keep two bottles of nanno going. One is aerated and is the main one I split and use for rotifers. The other bottle is not aerated. I just drilled a hole in the lid to allow for some gas exchange. I used to remember to "shake" it once or twice a day, but now I really only do it about once a week. This culture is never dense, but can be used as a back-up and/or "brought online" if I need to ramp up production. Years ago I cultured nearly all of my nanno this way...not aerated with a pump, just in 2L bottles that got manually mixed daily. Those bottle were also kept in a southern facing window with no addition light. I cultured that way for a year or so with little problem and those cultures were more dense because I actually paid attention to them
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<message edited by shannpeach on Tuesday, August 6, 2013 8:48 AM>