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Nannochloripsis
Monday, April 15, 2013 6:15 PM
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Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Phyto Species: Nannochloripsis Species description: Very dense Culture source (link if possible ): Original culture was from another hobbyist If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: End of Feb. 2013 Continuation Date: (Batch 1) 4/15, 4/29, ?, 5/29 (Batch 2) 4/22, 5/5, 5/26, split dates are no longer consistent and for me at least this is working Culturing Vessel Details Salinity: 1.019 Temperature: room temp pH: unknown
Vessel description: 2 1/2 L bottles Lighting description: 6,500 K T-5 Lighting cycle: 6am-10pm Aeration description: Good strong bubble Methodologies Split methodology: It was suggested to me that I should wait a month or 2 to split for denser cultures. They are being split monthly. I take 2 bottles when I split, one of finished product and one to be split between 3 new bottles. I have let bottles run up to a month and a half without issue. Culture medium description: Micro Algae grow10 drops per litter now changed to 5 drops of F/S per 2 littler or 3 mls of miracle grow per the same. Must also include that I re add the fertilizer weekly to the bottles Cell count: (if known) Reference links: his culture came from a hobbyist and he was running them up to 4months before split. Additional Information (No Pictures or Videos in the Section Please) Notes: About 2 months ago I was having 3 out of 5 bottles crash on me, and tried everything from using tap instead of RO, changing the SG and adding a fan foe air flow and better PH. urns out non of this was the problem and as soon as I started adding less fertilizer more often all my crashes stopped. Updated pictures below You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by TanksEveryWhere on Wednesday, October 23, 2013 12:38 PM>
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Re:Nannochloripsis
Monday, April 15, 2013 6:17 PM
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Re:Nannochloripsis
Monday, April 15, 2013 9:31 PM
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Those cultures are very dark!
I wouldn't split based on time but rather how dense/dark. I would be concerned that one day too long and your cultures could crash out.
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Re:Nannochloripsis
Monday, April 15, 2013 10:31 PM
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That's what I would assume, these cultures are very dark. I'm going to split a few off I think and see how long it takes one from start to crash.
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Re:Nannochloripsis
Monday, April 15, 2013 11:35 PM
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I just let my Iso get too dark and it crashed. Now I'm having a hard time starting it back up. Is it possible or do I need to get a new culture start?
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Re:Nannochloripsis
Tuesday, April 16, 2013 12:53 AM
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I tried starting my last one back up after it crashed and didn't have any luck. That was a FAF disk and this is a culture I got from a local. The local culture is much better, and I never got the other one close to as dense. I would see if anyone local has some you could get.
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Re:Nannochloripsis
Tuesday, April 16, 2013 8:46 AM
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 Originally Posted by nrbelk
I just let my Iso get too dark and it crashed. Now I'm having a hard time starting it back up. Is it possible or do I need to get a new culture start?
I have had luck sieving the Iso with a 40uM mesh and then restarting...but it was done before it went completely clear. It's worth a shot, but I wouldn't bank on it.
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Re:Nannochloripsis
Tuesday, April 16, 2013 10:58 AM
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I personally split my cultures on a 7 day cycle. If they have not reached full density within 7 days, then I know the culture is struggling. I have done this 7 day cycle for multiple types of phyto, and have not had any problems.
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Re:Nannochloripsis
Tuesday, April 16, 2013 11:02 AM
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That's how I was doing it w/ my last try. It was also The FAF disk, so not sure if that was it or not. Cultures were strong in the beginning, but that only lasted 3 months.
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Re:Nannochloripsis
Monday, April 22, 2013 3:14 PM
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So after splitting and giving a culture away last week I was left w/ 3 going. Than my kitties knocked one over and I had to fix their mistake. I'm left w/ 1 culture I started on the 15th and one started today. 
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Re:Nannochloripsis
Friday, April 26, 2013 9:57 PM
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So if I am running cultures for an extended amount of time, should I top them off?
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Re:Nannochloripsis
Friday, April 26, 2013 10:21 PM
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What do you mean, top off...like for evaporation? I keep my containers with lids or some other filter/blocking to limit cross contamination, and that limits evaporation.
Edit: I just looked at your pics. Are you really getting a lot of evaporation with lids on? How long between splits?
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Re:Nannochloripsis
Friday, April 26, 2013 10:26 PM
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They are lidded, but yes for evaporation as I will be keeping them going for closer to a month and there is some evap over that time.
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Re:Nannochloripsis
Friday, April 26, 2013 10:36 PM
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2 weeks to a month
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Re:Nannochloripsis
Friday, April 26, 2013 10:59 PM
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If they are dark I would split them.
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Re:Nannochloripsis
Friday, April 26, 2013 11:04 PM
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They are not as dark as I would like and I am trying out some advice from the person I got the culters from. Believe this or not, they have been running theirs for up to 4 months before splitting them and they are some of the best cultures I've seen.
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Re:Nannochloripsis
Monday, April 29, 2013 2:50 PM
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Split today, I think I am going to stick to a 2 week split scheduled, they are very dark at the 2 week mark and I probably could get a few more days out of them. I'll save that experiment for when I have a large supply though. Finished product  New cultures 
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Re:Nannochloripsis
Sunday, June 2, 2013 10:27 PM
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As of right now I have forgotten one of the dates from batch 1 but have since split again. I also did something a little different w/ each batch. Instead of just using the hobby F/S I split batch 2 w/ miraclegrow , and Batch 1 w/ the normal stuff. Dates are 3 days apart here is the result Batch 1 Hard to see but there is a huge difference  Batch 2 You can see one of the bottles from the other batch on the right] 
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Re:Nannochloripsis
Monday, October 7, 2013 10:20 AM
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Not running them as long as I would like or getting them as dark as I want, but it is a work in progress 
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Re:Nannochloripsis
Tuesday, October 8, 2013 1:40 PM
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Just curious: You seem to equate "dark" with "good". Why? I equate "nutritious" with "good".
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