Nannochloripsis

General Phyto
Species:  Nannochloripsis
Species description:  Very dense
Culture source (link if possible):  Original culture was from another hobbyist
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  End of Feb. 2013
Continuation Date:  (Batch 1) 4/15, 4/29, ?,  5/29
                   (Batch 2)  4/22, 5/5, 5/26,  split dates are no longer consistent and for me at least this is working
Culturing Vessel Details
Salinity:  1.019
Temperature:    room temp
pH:  unknown

Vessel description:  2 1/2 L bottles
Lighting description:  6,500 K T-5
Lighting cycle:  6am-10pm
Aeration description:  Good strong bubble

Methodologies
Split methodology: It was suggested to me that I should wait a month or 2 to split for denser cultures. They are being split monthly.  I take 2 bottles when I split, one of finished product and one to be split between 3 new bottles. I have let bottles run up to a month and a half without issue.

Culture medium description: 
Micro Algae grow10 drops per litter now changed to 5 drops of F/S per 2 littler or 3 mls of miracle grow  per the same. Must also include that I re add the fertilizer weekly to the bottles

Cell count:
 (if known)

Reference links:  
his culture came from a hobbyist and he was running them up to 4months before split.
Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
About 2 months ago I was having 3 out of 5 bottles crash on me, and tried everything from using tap instead of RO,  changing the SG and adding a fan foe air flow and better PH. urns out non of this was the problem and as soon as I started adding less fertilizer more often all my crashes stopped.

Updated pictures below

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And some pictures

That's what I would assume, these cultures are very dark. I'm going to split a few off I think and see how long it takes one from start to crash.

I just let my Iso get too dark and it crashed.  Now I'm having a hard time starting it back up.  Is it possible or do I need to get a new culture start?

I tried starting my last one back up after it crashed and didn't have any luck. That was a FAF disk and this is a culture I got from a local. The local culture is much better, and I never got the other one close to as dense. I would see if anyone local has some you could get.

I have had luck sieving the Iso with a 40uM mesh and then restarting...but it was done before it went completely clear.  It's worth a shot, but I wouldn't bank on it.

I personally split my cultures on a 7 day cycle.  If they have not reached full density within 7 days, then I know the culture is struggling.  I have done this 7 day cycle for multiple types of phyto, and have not had any problems.

That's how I was doing it w/ my last try. It was also The FAF disk, so not sure if that was it or not. Cultures were strong in the beginning, but that only lasted 3 months.

So after splitting and giving a culture away last week I was left w/ 3 going. Than my kitties knocked one over and I had to fix their mistake. I'm left w/ 1 culture I started on the 15th and one started today.
 

So if I am running cultures for an extended amount of time, should I top them off?

What do you mean, top off...like for evaporation? I keep my containers with lids or some other filter/blocking to limit cross contamination, and that limits evaporation.

Edit: I just looked at your pics. Are you really getting a lot of evaporation with lids on? How long between splits?

They are lidded, but yes for evaporation as I will be keeping them going for closer to a month and there is some evap over that time.

2 weeks to a month

If they are dark I would split them.

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

They are not as dark as I would like and I am trying out some advice from the person I got the culters from. Believe this or not, they have been running theirs for up to 4 months before splitting them and they are some of the best cultures I've seen.

Split today, I think I am going to stick to a 2 week split scheduled, they are very dark at the 2 week mark and I probably could get a few more days out of them. I'll save that experiment for when I have a large supply though.
Finished product

 
New cultures

As of right now I have forgotten one of the dates from batch 1 but have since split again. I also did something a little different w/ each batch.  Instead of just using the hobby F/S I split batch 2 w/ miraclegrow , and Batch 1 w/ the normal stuff.
 
Dates are 3 days apart here is the result
 
Batch 1
Hard to see but there is a huge difference

 
Batch 2
You can see one of the bottles from the other batch on the right]

 

Not running them as long as I would like or getting them as dark as I want, but it is a work in progress
 

Just curious:  You seem to equate "dark" with "good".  Why?  I equate "nutritious" with "good".

I'm not meaning dark as nutritious, I can see that it is that by the health of what it is being fed to and assume (which is more than likely a mistake) that the F/s and light are providing the nutrition. I use it for many things, but when I use it to tint the water my clown larval are in I want it as dense as I can get it. I have not found a sieve at a reasonable price to separate the phyto from the culture water. Dark/ dense is being used to refer to the fact that the culture is more phyto, less water and fertilizer.

With Nano, you can let a bottle settle in the fridge, and it will still be good for larval rearing, but you can decant off the clear liquid, and you are left with a much more concentrated phytoplankton.  No need to stress the culture trying to get it darker. Like most growing things, they will plateau and become senescent if left too long without suplemental fertilizer.  A 7 to 10 day schedule of splits has been best for me in my experience.  Miracle grow is a terrestrial plant fertilizer, so …it may not be the best choice to feed the food that feeds your larvae. Just my opinion.  Your results may vary.

check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)