Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis
Species description: green, motile, 10uM to 14uM
Culture source (link if possible
): Florida Aqua Farms;
http://florida-aqua-farms.com/shop/microalgae-disks/ If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 4-16-2013 (culture was actually started in August of 2012)
Continuation Date: 7-24-2013
Culturing Vessel Details Salinity: 1.020
Temperature: Ambient room temperature (68-72 in the winter, warmer during sunny days and spring/summer)
pH: not measured
Vessel description: 2L soda bottle
Lighting description: Ambient light from southern facing window, 2-bulb fluorescent strip light
Lighting cycle: Strip light is turned on from 6pm to 9pm when the days are shorter; not turned on during the summer
Aeration description: rigid airline tubing, rolling bubbles
Methodologies Split methodology: 2/3 of the culture is taken and replaced with sterilized, fertilized broodstock tank water every 4 to 10 days, depending on density. Then changed to using freshly mixed ASW instead of broodstock water
Culture medium description: 1/2mL FAF Microalgae grow per L
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Water is sterilized using bleach and dechlorinated using sodium thiosulfate
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Tuesday, August 6, 2013 8:46 AM>