Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Spirulina major
Species description: Blue-green algae (Cyanobacteria), spiralled trichomes
Culture source (link if possible
): Carolina Biological supply
If algae, CCMP # (Optional
): 1475/3
http://ccmp.bigelow.edu/ Culture Establishment Date: 4/17/2013
Continuation Date: Culturing Vessel Details Salinity: 1.020
Temperature: Room temperature
pH: Not measured
Vessel description: 500mL plastic container
Lighting description: Compact fluorescent bulb
Lighting cycle: Constant
Aeration description: Slow bubble
Methodologies Split methodology: During early culture, the thin film-like growth is sucked off the bottom with a bulb pipet.
Culture medium description: Sterilized, fertilized water. Fertilization is 1/2mL per L of water using FAF MicroAlgae Grow. Sterilization is done using bleach and sodium thiosulfate. Fertilization is 1/2mL per L of water using FAF MicroAlgae Grow into sterilized ASW. Sterilization is done using 1/2mL per liter of bleach for at least an hour and 1.5mL per liter of 1M sodium thiosulfate.
Cell count: (if known)
Reference links: http://www.algaebase.org/search/species/detail/?species_id=24748 Additional Information (No Pictures or Videos in the Section Please) Notes: Water is sterilized using bleach, and dechlorinated using sodium thiosulfate.
This culture seemed to do best with near constant light (but not very bright and directly on it) and very slow bubbling. It also did pretty well growing in petri dishes with no flow at all. Eventually I shoved the cultures to the side one day when I needed the spot they were in for a bit and just forgot to move them back and they died out over a week or so in the dark
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Wednesday, September 25, 2013 6:54 PM>