Culture Journal, Species: Isochrysis galbana

General
Species:  Isochrysis galbana
Species description:  golden-brown, motile, 1uM wide x 4-8uM long
Culture source (link if possible):  Fellow hobbyist
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  4/18/2013
Continuation Date:  7/24/2013

Culturing Vessel Details
Salinity:  1.020
Temperature:    Ambient room temp, currently ~72F
pH:  Not measured

Vessel description:  2L soda bottles
Lighting description:  Ambient light from southern facing window supplemented with 2-bulb fluorescent strip light
Lighting cycle:  Sunrise to sunset, strip light is on from 6PM to 9PM
Aeration description:  rolling bubble from rigid airline tubing

Methodologies
Split methodology:  Once culture is dark enough (I don't wait until it is "chocolate brown," I split when it is more of a dark "gold" color) I harvest 2/3 of the bottle and replace with sterilized, fertilized broodstock tank water.  Then changed to using sterilized freshly mixed ASW since it is easier to prepare.

Culture medium description: 
1/2mL of Florida Aqua Farm's Microalgae grow per 1L of sterilized water

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
Water is sterilized using bleach and neutralized using sodium thiosulfate


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The gold bottle in the center of the picture and the one on the far left are the two T-Iso bottles I currently have. 

 
I just reworked my culture area a bit and now I can expand the number of T-Iso bottles I have to at least three, possibly four.  This culture was actually started several weeks ago, but I am just getting around to making this culture journal now.  I have some microscope pics from last night, I just need to make sure I put them on my phone and will post them shortly if I have.

90+ days later and I still have the Iso going.  In the summer it seems to be a bit more difficult to maintain, but it's been hanging in there:

I am working on moving all three bottles to the 3L instead of using 2L, but I hate to just buy the water bottles and then waste the water so I need to drink a lot of water before I will have enough bottles.
 
The only thing I have changed is that usually now I just use freshly mixed ASW instead of re-using broodstock water.  It's more because I have been too lazy to sieve the broodstock water to get rid of the junk and I hate having buckets all over in various stages of phyto water prep.  I still sterilize the freshly mixed ASW using the same method I always have (bleach and sodium thiosulfate).