I use the word "minor" loosely because I started off with 1200 Ocellaris larvae and have much, much less now. However, there hasn't been a day where I saw a more significant die-off than other days other than one day when I had an ammonia spike (1200 babies requires
a lot of ClorAm-X). This daily die-off is annoying though, I only siphon out 10-20 every evening, but obviously many more are dying (they are almost 2 weeks now). Now, this problem isn't specific to this batch of Clowns. This is something I have struggled with for all the batches and species so far.
Here is what I do, let me know if there are details missing...
Hatch night I collect the tile, scrub it down (over the sink) everywhere there aren't eggs. Soak eggs in 1 liter of new ASW with 1-5 mL hydrogen peroxide (I've seen no difference in the amount, so I'm currently doing 5 mL) for 15 minutes, sometimes 30 minutes. Time doesn't seem to matter as far as initial hatch goes. The tile usually goes into the larvae tank (5-10 gallon tanks) that has been up and running with new ASW with 1 mL bleach per gallon for 12-24 hours, then dechlorinated for about 1 hour before eggs are introduced. I have checked chlorination to be sure I am adding enough thio. SG 1.020 and temp 80F (same as broodstock). I have tried using micron filtered broodstock water and have had total loss on the 3 occasions I have tried. I have messed around with aeration and find no difference with light aeration or heavy aeration.
Next morning I add rotifers and RGreen Omega. I add few enough rotifers that the larvae clear the tank about 95% by light out in the evening. I add about 1 mL RG Omega per 10 gallons. The morning after that I add maybe 0.5 mL RG Omega per 10 gallons to maintain greenwater, and add fresh rotifers. I continue this. Lighting is 7.5 watt incandescent bulb that shines on half the tank. I watch the fry and if they are avoiding the light I turn it away a bit more.
On day 3 I start adding a very small amount of TDO A a few times per day. The evening of the 3rd day is also the first watechange. I siphon out 50% through a coffee filter so any rotifers are removed, but larvae aren't sucked up. I bottom siphon this day too.
Around day 5-7 I switch out the 7.5 watt bulb for a 13 watt spiral compact fluorescent bulb which is quite bright. I shine it away from the tank so half is lit. When I find most of the larvae congregate under the lighting (close to meta) I turn it more towards them.
On days 6-9 I find are the worst for ammonia spike, I have to be really on top of it. Maybe this is a sign that I am losing many larvae at this point? Meta is usually a few days behind what I have read in other peoples' reports. Once I start seeing white bars on their heads they are eating TDO A quite well, and I start backing off the rotifers so I add just a small amount of rotifers twice per day that are usually cleared from the tank in a couple hours.
After meta, I usually lose 1-3 per day until around a month old. After a month it is unusual to lose a fry.
Any ideas??