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Culture Journal, Species: Tisbe biminiensis
Thursday, May 9, 2013 12:35 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Tisbe biminiensis Species description: Harpacticoid Culture source (link if possible ): Algagen via Seahorse Source If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 5/8/2013 , again on 4/17/14 Continuation Date: 7/16/14 Culturing Vessel Details Salinity: 27 ppt Temperature: ambient 78F in summer, and 65 in winter and everything in between pH: 8 Vessel description: 13x9x3 inch disposable food storage box with lid. Holes are punched in lid to allow air even when closed Lighting description: ambient Lighting cycle: 14 hours day, 10 hours night Aeration description: whatever can get in the hole unaided. Methodologies Split methodology: Ideal: Weekly: pour contents through 120 micron nested with 27 micron filters to capture adults and nauplii separately. Return most nauplii and some of the adults into clean new saltwater with live Isochrysis, and feed the rest to fish. Actual: feed live isochrysis and tetraselmus and slight sprinkling of dry spirulina every few days. Maybe do a water change once a month. These were not a high priority pod for me, so I put in very little effort. When needed, I dipped a container into the culture to collect some, and poured that through a 150 micron filter to capture adults, returning the nauplii and water to the culture. Culture medium description: 27 ppt saltwater, live isochrysis/tetraselmis/dry spirulina as needed every few days. Just enough to tint the water slightly. Cell count: unknown (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: The first culture I purchased was dead on arrival. The replacement culture was nearly so(2 day FEDEX, Seahorse Source send to me at no further charge). I could find many many live twitchers amongst a few dead bodies. I put them in about 200 ml clean water, tinted lightly with isochrysis, and let them go for about 2 weeks. Recently, when checking with a flashlight, there appears to be a loads of little twitchers of all sizes, so I think that the culture is established. Pictures below. I've already poured some off to feed the gobie larvae, and there are still loads left. Then that culture died. Started new culture in a larger culture pan. Still kind of struggling to keep it going, as i am unsure how much to feed. I think weekly water exchanges may be the way to go. Later I did not do weekly water changes , more like monthly ones, if that. I fed every few days, removed pods as i needed them, and I didn't need them much. I was very lazy with these, and they rewarded me by limping along and not dying. You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Saturday, February 7, 2015 12:21 PM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Tuesday, May 21, 2013 8:05 PM
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but now they are all dead. I am not great at Harpacticoids. Not good at Calanoids, but really not great at Harpacticoids...
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Tuesday, May 21, 2013 11:41 PM
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before they died, I did get some pictures: Here's a video of the culture when it was good: I think you have to click on it The iodined Tisbe:
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Wednesday, May 22, 2013 12:07 AM
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Kathy, how long did it take your's to die out? I got some about a month ago, and have been raising them identical to my rotifers, and they have been growing just about as fast. If I can keep them going like this, I think Tisbe would be great for feeding larvae just before they morph, and from the size of them, they look to be just a little larger than rotifers but smaller than NHBBS, so a perfect size.
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Re:Culture Journal, Species: Tisbe biminiensis
Sunday, June 9, 2013 12:49 PM
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I think it took a week after the video. I did not treat them like rotifers. I did get a few to survive, and still have a few, but not a great culture at this point. I am focusing on other stuff right now.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Sunday, May 4, 2014 1:09 AM
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I am back in the saddle with Tisbe, but struggling to know how much to feed them. I seem to have a bloom of nauplii, but the adults are few and far between…. not sure.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Sunday, May 4, 2014 7:34 AM
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I am going to try to mimic this, I think http://www.sciencedirect..../pii/S0044848611007241 But I'm not sure I'll do water changes every other day. Probably more like 2x a week. Last time I tried these I didn't feed any dry foods for the adults, I only used live phyto and I didn't have fantastic results. So this time I will be feeding some pulverized flake as well.
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Re:Culture Journal, Species: Tisbe biminiensis
Sunday, May 4, 2014 2:50 PM
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Thanks for this. I had forgotten about that paper!
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Sunday, May 25, 2014 10:14 AM
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Well, I have given it another go. Got some tisbe from Algagen(Seahorse Source) and this time put them in the cake-pan sized plastic storage containers made by Ziplock, that I found at the grocery store. They survived, I'm feeding them live Isochrysis. The key, I think, is to do water changes. Weekly changes seem to stimulate a bloom. Recently I was talking to M. Pedersen about breeding freshwater angelfish. He told me that to make them grow fast, you have to do a lot of water changes, as they put out a hormone that inhibits growth. Remove the hormone, and they grow really fast. Perhaps it is the same with copepods. If they produce a growth, or reproductive suppressing hormone, removing it may increase fecundity.
<message edited by KathyL on Wednesday, May 28, 2014 11:20 PM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Monday, May 26, 2014 12:13 AM
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The growth inhibiting hormone sounds like an interesting theory, but I've never read of it in the literature. That may speak more to my limited reading than anything else. But if it works for you then there may be something to it. It is also possible that the buildup of nitrate or other low level toxin is causing lowered fecundity. I think I read that tisbe biminiensis is a tidepool harpacticoid. If so it should tolerate high nutrient and pollution levels. Maybe a combination of growth inhibiting hormones and water pollution are both at work.
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Re:Culture Journal, Species: Tisbe biminiensis
Monday, May 26, 2014 3:00 PM
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But Dave, at least one of those papers you gave the reference to suggests that this happens in copepod culture. I'll look for it. later.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Monday, May 26, 2014 7:21 PM
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Kathy, it appears you have better memory retention that I do, because I don't remember seeing that in the literature. There is a paper in the copepod book dealing with the tendency of diatoms to act as birth controls, but I don't think that has much carryover at our small scale.
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Re:Culture Journal, Species: Tisbe biminiensis
Wednesday, May 28, 2014 11:22 PM
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Originally Posted by dave w
Kathy, it appears you have better memory retention that I do, I sincerely doubt that…. Here's my Tisbe picture:
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Friday, January 30, 2015 11:23 PM
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Well, I still have that culture going, even though it is not thriving, it is going, and here is a vid I took this evening. On the video, I say the date is 1/30/2014, but I meant to say 2015….It's OK, I'm old. Click on the box below. I should mention, that my culturing skills are not what they could be, in that I did not do weekly water changes. I did very few water changes, and only when i noticed the culture in decline. I don't actually have a great need for these pods, so I didn't put a lot of effort into them. Anyway, the continuation date is 6 months ago, but I've had the culture all this time, so I think it should still count.
<message edited by KathyL on Friday, January 30, 2015 11:36 PM>
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Friday, January 30, 2015 11:38 PM
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This evening I wanted to check that pods I have are still tisbe, and not some other copepod, so I did a quick google, and was linked back to one of my own photos. sad.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tisbe biminiensis
Thursday, June 4, 2015 1:57 AM
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Originally Posted by KathyL
This evening I wanted to check that pods I have are still tisbe, and not some other copepod, so I did a quick google, and was linked back to one of my own photos. sad. Priceless!
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