Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tisbe biminiensis Species description: Harpacticoid
Culture source (link if possible
): Algagen via Seahorse Source
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 5/8/2013 , again on 4/17/14
Continuation Date: 7/16/14
Culturing Vessel Details Salinity: 27 ppt
Temperature: ambient 78F in summer, and 65 in winter and everything in between
pH: 8
Vessel description: 13x9x3 inch disposable food storage box with lid. Holes are punched in lid to allow air even when closed
Lighting description: ambient
Lighting cycle: 14 hours day, 10 hours night
Aeration description: whatever can get in the hole unaided.
Methodologies Split methodology: Ideal: Weekly: pour contents through 120 micron nested with 27 micron filters to capture adults and nauplii separately. Return most nauplii and some of the adults into clean new saltwater with live Isochrysis, and feed the rest to fish.
Actual: feed live isochrysis and tetraselmus and slight sprinkling of dry spirulina every few days. Maybe do a water change once a month. These were not a high priority pod for me, so I put in very little effort. When needed, I dipped a container into the culture to collect some, and poured that through a 150 micron filter to capture adults, returning the nauplii and water to the culture.
Culture medium description: 27 ppt saltwater, live isochrysis/tetraselmis/dry spirulina as needed every few days. Just enough to tint the water slightly.
Cell count: unknown
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: The first culture I purchased was dead on arrival. The replacement culture was nearly so(2 day FEDEX, Seahorse Source send to me at no further charge). I could find many many live twitchers amongst a few dead bodies. I put them in about 200 ml clean water, tinted lightly with isochrysis, and let them go for about 2 weeks. Recently, when checking with a flashlight, there appears to be a loads of little twitchers of all sizes, so I think that the culture is established. Pictures below.
I've already poured some off to feed the gobie larvae, and there are still loads left.
Then that culture died.
Started new culture in a larger culture pan. Still kind of struggling to keep it going, as i am unsure how much to feed. I think weekly water exchanges may be the way to go.
Later I did not do weekly water changes , more like monthly ones, if that. I fed every few days, removed pods as i needed them, and I didn't need them much. I was very lazy with these, and they rewarded me by limping along and not dying.
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<message edited by KathyL on Saturday, February 7, 2015 12:21 PM>