Culture Journal, Species: Colurella adriatica

General
Species:  Colurella adriatica
Species description:  Tiny rotifer.  A ciliated protozoan.
Culture source (link if possible):  Eric Cassiano and his colleagues at the Tropcial Aquaculture Laboratory
If algae, CCMP # (Optional):  N/A
http://ccmp.bigelow.edu/
Culture Establishment Date:  6/1/2013
Continuation Date: 

Culturing Vessel Details
Salinity:  35 PPT / 1.026 SG
Temperature:    Upper 70s F
pH:  Not Measured

Vessel description: Several 1000 ml Erlenmeyer flasks
Lighting description:  N/A - Ambient fish room light
Lighting cycle:  N/A - Ambient fish room light cycle
Aeration description:  Appx. 1 bubble per second from a rigid airline.

Methodologies
Split methodology:

Culture medium description: 
ASW at 35 PPT

Cell count:
  Not known or measured.  Very dense, relative to my other rotifer cultures.

Reference links:   http://risingtideconserva...aquaculture-lab_7.html (search for "Colurella")

Additional Information
Notes:  They arrived in a co-culture together with the copepod Oithona colcarva.  I will attempt to isolate a pure Colurella culture at a later date.

Is this the rotifer that Eric talked about at the conference last year? I tried finding my original notes from the conference but cannot find them at the moment.

Yes, it is.

I got some crappy images using the scope in the lab today.  I was in a hurry.  Sorry for the poor quality.
 
These rotifers are tiny, and are also very flattened in shape.  There is a "wide" aspect, and a "thin" aspect.  I managed to get some rough measurements from both angles.
 
100x:

 
100x, measured in the thin dimension:

 
400x, measured in the wide dimension and length:

 
 
 
 

Attached Image(s)

Awesome pics!

Do you have something specific planned for these little guys?

Hi Jim, how are you doing with these?  What food are you using, are they as easy as other more common types of rotifers?

Proud member of the first dedicated breeders site in the UK
Marine Fish Breeders Club - UK
H.Zosterae - 60 DPS

Any updates on these Jim?

Yes, updates would be great.  Any luck with the culture?

The culture is still alive, but not thriving.  I'm currently at MACNA 2013, and got some great tips from Matt Wittenrich on culturing them that I'll try once I get back home.

Nice! Good luck with them.

--Andy, the bucket man.
"Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886

Hey Jim;
Hope all is well. I have been researching both the C.Adriatica and Oithona Colcarva as possible prey items for Midas Blenny Larvae. L-type rotifers haven't worked on several occasions, larvae die in about first 48 hrs. I am going to try B. Rotundiformis I am gonna to order from ReedMariculture and give them a try. Wondering if your culture(s) of forementioned are doing? Have you been able to isolate them? And lastly, if they are thriving, would you please share some , or tell me where I could get some? Thanks.
 
Rich from Miami
 

would also like an update Jim... how are these doing?
 

Jim--what were the tips (if you can tell us)?  I think I may have found some of these in a larval "tank" of my Stenopus and want to try to get a culture going if I can.

Any updates on these ?

Jake
We are all in this together