Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Species description: Culture source (Local): If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 31/5/2013
Continuation Date: 6/6/2013
Culturing Vessel Details Salinity: 1.020 - 1.022
Temperature: 24-26 deg C
pH: 8.5
Vessel description: First established in 1.5L plastic bottle, then moved to 2x 1L glass vessels
Lighting description: T5 tubes x2 (24W)
Lighting cycle: 16 light - 8 dark
Aeration description: Moderate, near boiling
Methodologies Split methodology: culture was split on the 5th or 6th day or when it gets to a darkish milk chocolate colour, splitting it before it gets too dark to avoid risking a crash.
the culture was split to by a 50% ratio, (will probably do 1:3 later to increase production speed)
The new culture water was microwaved for 3 minutes or till its just about to boil then left to cool before used. glass vessels and tubing were left in boiling water before cooled to room temperature.
Lids are covered with plastic film and a small hole is left for air to escape.
Culture medium description: 10 drops of FAF microalgae grow per 1L culture.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: This is my second attempt at ISO, the 1st time was a total failure with the FAF disc, it just never coloured up.
This starter came from a local store and it came in a light color, altho it eventually darkened up to the ideal color.
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<message edited by Tomngangan on Thursday, June 6, 2013 3:59 AM>