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Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:00 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Nannocloropsis oculata Species description: non motile green single cell 1-2µ in size. Culture source (link if possible ): Fellow hobbyist. If algae, CCMP # (Optional ): https://ncma.bigelow.org/node/1/strain/CCMP525 http://ccmp.bigelow.edu/ Culture Establishment Date: 04/06/2013 Continuation Date: current ongoing culture. Culturing Vessel Details Salinity: 1.019-1.022 Temperature: 76-78 pH: 7.5-7.9 Vessel description: 2L bottles Lighting description: 1 compact bulb Lighting cycle: 16 on, 8 off Aeration description: vigorous bubbling per rigid tubing. Methodologies Split methodology: Keep 1/5-1/4 of the culture in original container, pouring off/using off the remainder. Fill 2L with fresh salt water and add Fritz brand F2 formula (Part A and B) 0.25mL of each to the 2L bottle. Culture medium description: (this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” ) Cell count: dark! (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: I specifically chose Fritz two part solution because the vitamins and minerals are stored separated...this enables a longer shelf life of the product! You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:10 AM
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I am finally getting around to posting this journal! So, I got a starter from a local fellow hobbyist, and began taking the plunge of live algae culturing. I started with nanno due to it's easy reputation- quickly finding out it is just that. Began culturing with the Miracle Grow + essential elements formula (2L bottle with 0.5mL of mg and 0.2mL EE). Have since then switched to F2 (Fritz 2 part solutions). ***NOTE: Miracle grow and essential elements WILL grow this algae, and quite rapidly, is sufficient at keeping LOW density rotifers alive (NOT thriving)... The rotifers fed off this have such a poor nutrient profile they are unable to sustain fry for extended lengths of time.
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:23 AM
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Got all my culture vessels switched entirely (100%) to F2 this past week! I also realized I never posted any pictures of this setup! It is in a cedar box, with a white board top, along with rotifer and brine culturing. Roti's and brine are not covered, but all algae's are sealed.
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:28 AM
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Here's a full shot of the box it's in. Makes it to where I can shut the lid and hear practically nothing!
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:32 AM
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Chicken scratch notes to myself for cultures... splits/crashes, restarts, who and what I fed... Thank God for that white board! lol
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:35 AM
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Today is technically day 87, for this culture.... doing good so far! I have crashed several 2L but never too far gone! Need to get the scope out and get some good close ups of this culture! Will do this in the morning!
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 8:14 AM
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What is Fritz Brand f2? Never heard of it, and it doesn't pop up on google...
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 8:19 AM
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Re:Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 8:22 AM
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My Chemist husband refuses to let me get the mixed batch of F2, (meaning vitamins and minerals are already in the same container, not separated). It's an extra step for me, but hardly one worth noting. And he thinks this will have a more stable and longer shelf life. And since he foots the bill... I am humoring him on this one!
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Re:Culture Journal, Species: Nannocloropsis
Wednesday, August 14, 2013 5:19 AM
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