Culture Journal, Species: Nannocloropsis

General
Species:  Nannocloropsis oculata
Species description:  non motile green single cell 1-2µ in size. 
Culture source (link if possible):  Fellow hobbyist. 
If algae, CCMP # (Optional):  https://ncma.bigelow.org/node/1/strain/CCMP525
http://ccmp.bigelow.edu/
Culture Establishment Date:  04/06/2013
Continuation Date:  current ongoing culture. 

Culturing Vessel Details
Salinity:  1.019-1.022 
Temperature:    76-78
pH: 7.5-7.9

Vessel description:  2L bottles
Lighting description:  1 compact bulb
Lighting cycle:  16 on, 8 off
Aeration description:  vigorous bubbling per rigid tubing. 

Methodologies
Split methodology: Keep 1/5-1/4 of the culture in original container, pouring off/using off the remainder. Fill 2L with fresh salt water and add Fritz brand F2 formula (Part A and B) 0.25mL of each to the 2L bottle. 

Culture medium description: 
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )

Cell count: dark!
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes:  I specifically chose Fritz two part solution because the vitamins and minerals are stored separated...this enables a longer shelf life of the product! 



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I am finally getting around to posting this journal!  
 
So, I got a starter from a local fellow hobbyist, and began taking the plunge of live algae culturing. I started with nanno due to it's easy reputation- quickly finding out it is just that. 
 
Began culturing with the Miracle Grow + essential elements formula (2L bottle with 0.5mL of mg and 0.2mL EE). Have since then switched to F2 (Fritz 2 part solutions). 
 
***NOTE: Miracle grow and essential elements WILL grow this algae, and quite rapidly, is sufficient at keeping LOW density rotifers alive (NOT thriving)... The rotifers fed off this have such a poor nutrient profile they are unable to sustain fry for extended lengths of time. 
 
 

Got all my culture vessels switched entirely (100%) to F2 this past week! I also realized I never posted any pictures of this setup! 
 
It is in a cedar box, with a white board top, along with rotifer and brine culturing. Roti's and brine are not covered, but all algae's are sealed.  

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Here's a full shot of the box it's in. Makes it to where I can shut the lid and hear practically nothing! 

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Chicken scratch notes to myself for cultures... splits/crashes, restarts, who and what I fed... Thank God for that white board! lol
 

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Today is technically day 87, for this culture.... doing good so far! I have crashed several 2L but never too far gone! Need to get the scope out and get some good close ups of this culture! Will do this in the morning!

What is Fritz Brand f2? Never heard of it, and it doesn't pop up on google...

check out Kathy's Clowns, llc website:
http://kathysclowns.com
Captive bred clownfish and more
(Wholesale to the trade.)

http://www.fritzpet.com/algae-food/