Reports tied to this Journal
Culture Journal, Species: Nannocloropsis
Tuesday, June 25, 2013 3:00 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Nannocloropsis oculata Species description: non motile green single cell 1-2µ in size. Culture source (link if possible ): Fellow hobbyist. If algae, CCMP # (Optional ): https://ncma.bigelow.org/node/1/strain/CCMP525 http://ccmp.bigelow.edu/ Culture Establishment Date: 04/06/2013 Continuation Date: current ongoing culture. Culturing Vessel Details Salinity: 1.019-1.022 Temperature: 76-78 pH: 7.5-7.9 Vessel description: 2L bottles Lighting description: 1 compact bulb Lighting cycle: 16 on, 8 off Aeration description: vigorous bubbling per rigid tubing. Methodologies Split methodology: Keep 1/5-1/4 of the culture in original container, pouring off/using off the remainder. Fill 2L with fresh salt water and add Fritz brand F2 formula (Part A and B) 0.25mL of each to the 2L bottle. Culture medium description: (this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” ) Cell count: dark! (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: I specifically chose Fritz two part solution because the vitamins and minerals are stored separated...this enables a longer shelf life of the product! You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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