Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachonius rotundiformis
Species description: S strain rotifers
Culture source (link if possible
): Seahorse source
http://www.seahorsesource.com/cgi-bin/shop/detail.cgi?id=201140 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 9-5-2013
Continuation Date: Culturing Vessel Details Salinity: ~1.022 but plan to lower it
Temperature: Room temperature (70-80F)
pH: Not Measured
Vessel description: 1 L bottle to start, moved to one gallon container once established
Lighting description: Ambient light from southern facing window, supplemented by 2-bulb fluorescent strip light. Will move to the rotifer culture area once the culture is more established, which has a 2 bulb fluorescent strip light only.
Lighting cycle: 6 AM to 9 PM
Aeration description: Rigid airline, enough aeration to provide constant movement, but not a "rolling boil"
Methodologies Split methodology: Approximately once or twice a week 75-90% of the culture is dumped out and new, sterilized ASW is added. The entire culture is moved to a new/sterilized container once every two or three weeks
Culture medium description: Culture is fed live phyto (Nannochloropsis, Tetraselmis, Isochrysis, Rhodomonas, or Chaetoceros) or roti-rich (Florida Aqua Farms) once or twice daily (twice daily if the rotifer density is high) enough to tint the water slightly
Cell count: Not counted
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Thursday, December 5, 2013 9:12 AM>