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Culture Journal, Species: Rhodomonas lens
Friday, September 6, 2013 9:20 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Rhodomonas lens Species description: Marine cryptomonad alga common to the baltic regions, popular species for copepod rearing. Cells are oval and relatively flat with two flagella protruding and red plastids. Culture source (link if possible ): Seahorse source http://www.seahorsesource.com/cgi-bin/shop/detail.cgi?id=201133 If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 9-5-2013 Continuation Date: Culturing Vessel Details Salinity: ~1.022 Temperature: Room temp (70-80F) pH: Not measured Vessel description: 1 L bottle to start for scaling up, eventually hope to have it in 3L containers Lighting description: Ambient light from a southern facing window, supplemented by a 2-bulb fluorescent strip light Lighting cycle: 6 AM to 9 PM Aeration description: Rigid airline, pretty vigorous right now. Methodologies Split methodology: Culture medium description: 1/2mL per liter of Florida Aqua Farm's Microalgae Grow in sterilized water (1mL of bleach per L to sterilize for at least an hour, following by 1.5mL 1M sodium thiosulfate per L to neutralize) Cell count: (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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