Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus rotundiformis (S-type rotifer)
Species description: small strain of rotifers (90-150 µm in size)
Culture source (link if possible
): http://www.reedmariculture.com/product_instant_zooplankton.php#rotifers If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: Sept. 10th, 2013 (09-10-2013)
Continuation Date: Culturing Vessel Details Salinity: 1.023
Temperature: ~74 F
pH: 8.2
Vessel description: 5g buckets. 1 5g bucket with compact culture kit from reed mariculture, one with DIY version of cck.
Lighting description: none
Lighting cycle: none
Aeration description: vigorous rolling of air bubles on single line, with glass/silicate airstone.
Methodologies Split methodology: once ever few days grab a small bucket of culture water and critters and use for feed off, otherwise let run until weekly culture split of 30%keep, 70% toss (or use)
Culture medium description: Rotigrow Plus 1-2ml every other day or so
Cell count: not known right now, need to do a count.
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.