Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Isochrysis galbana (T.Iso)
Species description: Motile brown/yellow golden algae ~ 5µm in size. (cell length of 4-6µm)
Culture source (link if possible
): Algagen via seahorsesource.com
If algae, CCMP # (Optional
): https://ncma.bigelow.org/node/1/strain/CCMP1323 http://ccmp.bigelow.edu/ Culture Establishment Date: 10-25-2013
Continuation Date: DUE for Jan. 23rd, 2014
Culturing Vessel Details Salinity: 1.020
Temperature: 72 F (room temp)
pH: 8.0
Vessel description: 1qt container canning jar, 300mL Erlenmeyer flask, 2L glass cylinders (x2).
Lighting description: 2 18" compact bulbs at 2600K
Lighting cycle: 24hr constant to ramp up culture, then I'll back it down to an 18hrs on & 6hrs off.
Aeration description: rolling.
Methodologies Split methodology: none yet.
Culture medium description: (this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )
Cell count: (if known)
Reference links: Referring to journals: JimWelsh and KathyL, also referring to planktopedia.org and NCMA (formally the CCMP).
Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by jazzybio13 on Wednesday, June 18, 2014 5:21 AM>