Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tigriopus californicus
Species description: Harpacticoid copepod
Culture source (link if possible
): I bought them at my LFS, but can't remember from what company
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 1-22-2014 (I've actually had this culture for 9 months or maybe a year...)
Continuation Date: 4-23-2014
Culturing Vessel Details Salinity: ~1.024
Temperature: Room temperature. Varies from 68-84 based on the time of the year
pH: Not measured
Vessel description: ~4L clear plastic containers at first for a long time, now use clear plastic shoeboxes
Lighting description: Ambient light from nearby fluorescent strip light and southern facing window
Lighting cycle: sunrise to sunset, supplemented by fluorescent strip light until 9PM
Aeration description: None
Methodologies Split methodology: When the population looks dense enough I harvest by siphoning them out, then replace the water I removed with sterilized ASW (1/2mL per liter bleach sterilized, 1 1/2mL per L of 1M sodium thiosulfate neutralized)
Culture medium description: The culture is kept continually green/brown with live phyto, often pretty "dark." I use motile algae that seem to settle less (Tetraselmis or Isochrysis) and usually only need to add phyto once or twice over a month or after water change/harvest.
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Wednesday, April 30, 2014 9:37 AM>