Reports tied to this Journal
Culture Journal, Species: Thalassiosira
Monday, January 27, 2014 10:09 AM
( permalink)
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Thalassiosira Species description: Thalassiosira is a marine culture with auxospores; relatively large at 10-15 µ wide by 10-20 µ long; diatom Culture source (link if possible ): http://www.carolina.com/algae/thalassiosira-living/153110.pr?catId=&mCat=&sCat=&ssCat=&question=thalassiosira%2C+living If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 1-24-2014 Continuation Date: Culturing Vessel Details Salinity: ~1.023 Temperature: Room temperature pH: Not measured
Vessel description: 3L bottle Lighting description: Fluorescent strip light Lighting cycle: 6AM to 9PM Aeration description: Rigid airline Methodologies Split methodology: Culture medium description: (this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” ) Cell count: (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
|
|
|
Re:Culture Journal, Species: Thalassiosira
Monday, January 27, 2014 10:10 AM
( permalink)
The starter culture:  After a couple days in a small 250mL bottle:  I mainly got this to try it as a potential feed for the Gymnodinium since I have read papers that show it prefers larger phytoplankton cells like tetraselmis
|
|
|
Re:Culture Journal, Species: Thalassiosira
Thursday, February 6, 2014 9:22 AM
( permalink)
Some microscope pics of the culture  
|
|
|
Re:Culture Journal, Species: Thalassiosira
Monday, March 10, 2014 4:43 PM
( permalink)
Well, I killed this culture over the weekend. Not the end of the world since it didn't work for what I wanted it for. I actually think it would be pretty easy to culture, assuming that you actually split it when needed...I sort of abandoned it and then when I finally did split it, it was already too far gone.
|
|
|