Culture Journal, Species: Thalassiosira

General
Species:  Thalassiosira
Species description:  Thalassiosira is a marine culture with auxospores; relatively large at 10-15 µ wide by 10-20 µ long; diatom  
Culture source (link if possible):  http://www.carolina.com/algae/thalassiosira-living/153110.pr?catId=&mCat=&sCat=&ssCat=&question=thalassiosira%2C+living
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  1-24-2014
Continuation Date: 

Culturing Vessel Details
Salinity:  ~1.023
Temperature:    Room temperature
pH:  Not measured

Vessel description:  3L bottle
Lighting description:  Fluorescent strip light
Lighting cycle:  6AM to 9PM
Aeration description:  Rigid airline

Methodologies
Split methodology:

Culture medium description: 
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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The starter culture:

After a couple days in a small 250mL bottle:

 
I mainly got this to try it as a potential feed for the Gymnodinium since I have read papers that show it prefers larger phytoplankton cells like tetraselmis
 

Some microscope pics of the culture

Well, I killed this culture over the weekend. Not the end of the world since it didn't work for what I wanted it for. I actually think it would be pretty easy to culture, assuming that you actually split it when needed...I sort of abandoned it and then when I finally did split it, it was already too far gone.