Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Dunaliella tertiolecta
Species description: "Cells permanently green, radially symmetrical, ellipsoidal, oval or rarely ovate or pyriform. Cells 5-18 µm in length (mean 9.412.4 m), 4.514.0 m wide (mean 7.1-8.2 µm)." - http://www.algaebase.org/search/species/detail/?species_id=51616
Culture source (link if possible
): http://florida-aqua-farms../shop/microalgae-disks/ If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: Continuation Date: Culturing Vessel Details Salinity: 1.028
Temperature: Ambient, around 75-78F
pH: Not Measured
Vessel description: 500ml canning jar
Lighting description: 3 Lights of America Linkable Fluorescent Undercabinet Portable Lights
Lighting cycle: 22 hours on, 2 hours off
Aeration description: Turbulent
Methodologies Split methodology: Every day, 25-50% split per culture
Culture medium description:
1. Add 2x Guillards F/2 formula per 100ml of culture water
2. Heat in microwave until 212F has been achieved
3. Allow cooling until room temperature
4. Split algae into containers
5. Run airline into culture with a mild-turbulent flow
6. Have warm lights on the culture running at 22 hours on, 2 hours off
7. Split every day or two, or until the jar has a light green tint to it
8. Repeat 2-6 for every split
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by Amphispur on Tuesday, March 11, 2014 9:57 PM>