Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Arctodiaptomus salinus
Species description: Calanoid copepod
Culture source (link if possible
): http://www.algagen.com/?p=114 If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 1-31-2014
Continuation Date: 5-1-2014
Culturing Vessel Details Salinity: ~1.021-1.024
Temperature: Room temp, 68-80F
pH: Not measured
Vessel description: 1 gallon container, clear
Lighting description: Fluorescent strip light, and ambient light from southern facing window
Lighting cycle: 6AM-9PM
Aeration description: Rigid airline, continuous bubble but not a "boil"
Methodologies Split methodology: When the copepods are needed, I pour part of the culture through a 53uM mesh and then replace the dumped water with new ASW.
Culture medium description: I used sterilized ASW (0.5 mL/liter bleach for at least 30 minutes, neutralized with 1.5mL/liter of 1M sodium thiosulfate) for culture water and add live phyto plankton (Isochrysis or Tetraselmis) as needed, just enough to tint the water slightly. Lately, I have been using RG complete (Reed) for feeding, and add a couple drops every other day or so.
Cell count: Not measured
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Wednesday, June 11, 2014 8:05 PM>