Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Oxyrrhis marina
Species description: Heterotrophic dinoflagellate
Culture source (link if possible
): If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 4-8-2014 (first photographed for establishment proof on 4-19-2014)
Continuation Date: Culturing Vessel Details Salinity: ~1.021
Temperature: ~64-70F (basement room temp)
pH: Not measured
Vessel description: 500mL bottle
Lighting description: Fluorescent strip light
Lighting cycle: 14 hours on, 10 off
Aeration description: Rigid airline tubing set to a continuous bubble, but not a boil
Methodologies Split methodology: I split every couple days, or as the culture seems to need it.
Culture medium description: A squirt of Iso or RG complete for feeding as needed, and split using ~1.022 (ish) ASW that has been sterilized (1/2mL bleach per liter for 1 hours, neutralized with 1.5mL of 1M sodium thiosulfate per liter)
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by shannpeach on Wednesday, April 30, 2014 12:16 PM>