Culture Journal, Species: Oxyrrhis marina

General
Species:  Oxyrrhis marina
Species description:  Heterotrophic dinoflagellate
Culture source (link if possible): 
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  4-8-2014 (first photographed for establishment proof on 4-19-2014)
Continuation Date: 

Culturing Vessel Details
Salinity:  ~1.021
Temperature:    ~64-70F (basement room temp) 
pH:  Not measured

Vessel description:  500mL bottle
Lighting description:  Fluorescent strip light
Lighting cycle:  14 hours on, 10 off
Aeration description:  Rigid airline tubing set to a continuous bubble, but not a boil 

Methodologies
Split methodology: I split every couple days, or as the culture seems to need it.

Culture medium description: 
A squirt of Iso or RG complete for feeding as needed, and split using ~1.022 (ish) ASW that has been sterilized (1/2mL bleach per liter for 1 hours, neutralized with 1.5mL of 1M sodium thiosulfate per liter) 

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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Finally got time for some pics. I had thought I lost the culture, but today I found some cruising around. The culture isn't "thriving" but it's still going at least

Took a peek at the culture again tonight and it is already more dense. I've been feeding Iso. Well, actually, I fed Iso days ago but it still hasn't all been eaten.

Definitely split these often. Their population doubling rate is really fast.

--Andy, the bucket man.
"Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886