| shannpeach 
                
                    
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                    Joined: 5/30/2012Location: Milwaukee, WI, US | 
                
                    
                        
                             Culture Journal, Species: Micromonas pusilla
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                            Thursday, April 10, 2014 9:06 AM 
                    
                    
                      Culturing Journal DataSheet  General     Species:This first post should be updated regularly to include new information as events take place or changes are made to your system
   Micromonas pusilla   Species description: picophytoplankton, usually less than 2uM   Culture source ( link if possible):              If algae, CCMP # ( Optional):        http://ccmp.bigelow.edu/    Culture Establishment Date:   4-8-2014   Continuation Date:         Culturing Vessel Details     Salinity:   ~1.021   Temperature:     ~65 F   pH:   Not measured Vessel description:
   500mL bottle   Lighting description:   Fluorescent strip light   Lighting cycle:   14 hours on, 10 hours off   Aeration description:  None at the moment      Methodologies     Split methodology:       Culture medium description:  0.5mL of FAF Microalgae grow per L of ASW (Sterilized with 0.5mL of bleach per L, neutralized with 1.5mL of 1M sodium thiosulfate per L)      Cell count:    (if known)                Reference links:                  Additional Information     (No Pictures or Videos in the Section Please)    Notes:   Got a very small starter of this.  We'll see how it does!      You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.
 
                    
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        | shannpeach 
                
                    
                        Total Posts
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                        955
                    
                        Reward points
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                        676
                    Joined: 5/30/2012Location: Milwaukee, WI, US | 
                
                    
                        
                             Re:Culture Journal, Species: Micromonas pusilla
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                            Thursday, April 10, 2014 9:07 AM 
                    
                    
                      This is M. pusilla using a 10x objective and you can still hardly see it!  It is SO small      The 40x pic isn't very good since the table wasn't steady enough to focus on something so small     
                    
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