Culture Journal, Species: Micromonas pusilla

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shannpeach
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Culture Journal, Species: Micromonas pusilla - Thursday, April 10, 2014 9:06 AM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Micromonas pusilla
Species description:  picophytoplankton, usually less than 2uM
Culture source (link if possible): 
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  4-8-2014
Continuation Date: 

Culturing Vessel Details
Salinity:  ~1.021
Temperature:    ~65 F
pH:  Not measured

Vessel description:  500mL bottle
Lighting description:  Fluorescent strip light
Lighting cycle:  14 hours on, 10 hours off
Aeration description:  None at the moment

Methodologies
Split methodology:

Culture medium description: 
0.5mL of FAF Microalgae grow per L of ASW (Sterilized with 0.5mL of bleach per L, neutralized with 1.5mL of 1M sodium thiosulfate per L)

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 

Got a very small starter of this.  We'll see how it does!

You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.


shannpeach
  • Total Posts : 955
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  • Joined: 5/30/2012
  • Location: Milwaukee, WI, US
Re:Culture Journal, Species: Micromonas pusilla - Thursday, April 10, 2014 9:07 AM
This is M. pusilla using a 10x objective and you can still hardly see it!  It is SO small

 
The 40x pic isn't very good since the table wasn't steady enough to focus on something so small