Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Pseudodiaptomus pelagicus Species description: medium sized calanoid copepod
Culture source (link if possible
): Algagen, via Seahorse Source
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 4/17/14
Continuation Date: 8/12/14
Culturing Vessel Details Salinity: 27ppt
Temperature: ambient, varies by season, but now about 74F
pH: 8
Vessel description: 4x4x4 inch clear plastic square jar, previously contained rice, with a drilled cap , then a plastic ziplock tray with lid, hole drilled in cap (then after the 90 day report, a 3 gallon black trash can from the dollar store). The trash can seemed to work best, but needed a lid
Lighting description: ambient
Lighting cycle: 14 hours day, 10 hours night
Aeration description: exposed to air through hole in cap
Methodologies Split methodology: split every month or so. It would have grown better if split weekly, I think.
Culture medium description: about 50 ml of weak tea colored live Isochrysis galbana culture 2-3 times a week at recent populations, or whenever clear.
Cell count: (if known) not known
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: By ambient lighting, I mean that no lighting was placed to facilitate copepod growth other than room lighting. Room lighting was a Compact Fluorescent Bulb, 40 watts, in a reflective fixture clamped to an exposed beam in the ceiling.
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<message edited by KathyL on Saturday, July 11, 2015 12:00 PM>