Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tetraselmis suecica Species description: This is a green, motile microalge with a high lipid content. Approximate cell size: 9-10 µ wide by 12-14 µ long.
Culture source (link if possible
): Seahorse Source
(
http://seahorsesource.com...50-ml-starter-culture) If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 10-25-2013
Continuation Date: Jan. 23rd 2014
Culturing Vessel Details Salinity: 1.020
Temperature: 72-74 F
pH: 8.0
Vessel description: Started out in a 300mL Erlenmeyer flask and a 2L glass cylinders (x2). Now I still keep the 300mL Erlenmeyer going as my starter, but also run the rest of the culture in 1g plastic jugs (just for ease of handling).
Lighting description: 2 18" compact bulbs at 2600K
Lighting cycle: 24hr constant to ramp up culture, then I'll back it down to an 18hrs on & 6hrs off.
Aeration description: 2-3 bubbles per second.
Methodologies Split methodology: take culture and split into thirds. 1/3 restart into new jug, 1/3 feed rotifers, 1/3 feed all the other cultures (or keep in water bottle till feedings are needed later that week).
Culture medium description: 2-3mL F2 (micro grow) fed at split.
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )
Cell count: (if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.